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- W2079192211 abstract "A cDNA clone containing a 921 bp open-reading frame (307 amino acids; 34 kDa) homologous to the TATA-binding protein (TBP) was isolated and sequenced from a Spodoptera frugiperda cell line that is commonly used in the baculovvrus expression system. Analysis of the S. frugiperda TBP (SfTBP) sequence showed that the amino-terminal portion of SfTBP diverged significantly from that of other TBP sequences including Drosophila melanogaster whereas the carboxy-terminal sequence was highly conserved. Southern blot analysis indicated that SfTBP was encoded by a single gene in the S. frugiperda genome. Northern blot analysis indicated that steady-state levels of the 1.3 kb SfTBP transcript declined by 24 h post-infection corresponding to the time of virus-induced inhibition of host-cell transcription. Corresponding western blot analysis showed that TBP protein levels remain constant up to 72 h post-infection." @default.
- W2079192211 created "2016-06-24" @default.
- W2079192211 creator A5052118011 @default.
- W2079192211 creator A5087751268 @default.
- W2079192211 date "1994-07-01" @default.
- W2079192211 modified "2023-09-25" @default.
- W2079192211 title "Characterization of the Spodoptera frugiperda TATA-binding protein: Nucleotide sequence and response to baculovirus infection" @default.
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- W2079192211 doi "https://doi.org/10.1016/0965-1748(94)90057-4" @default.
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