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- W2079213532 abstract "The number and spatial distribution of small positively-charged ions around highly negatively charged DNA or RNA contribute to the free energy of binding in vitro and in vivo. However, the majority of charge compensating ions around nucleic acids forms a diffuse counterion “cloud” that is not amenable to investigation by traditional methods that rely on rigid structural interactions. With 2 x-ray energies, one near and the other 100 eV away from the ion absorption edge, we have successfully used Anomalous Small-Angle X-ray Scattering (ASAXS) to compare and differentiate the ion spatial distribution around comparably sequenced short DNA and RNA helices. Here, we present further information gained when using multiple x-ray energies (up to 5) in an ASAXS experiment. We describe proper treatment of multiple-energy SAXS data including absolute SAXS intensity calibration and measurement of scattering factors from x-ray fluorescence. We discuss the strengths and limitations of this approach and derive useful parameters in depicting the nucleic acid ion atmosphere." @default.
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- W2079213532 date "2011-02-01" @default.
- W2079213532 modified "2023-09-28" @default.
- W2079213532 title "Characterization of DNA and RNA Ion Atmospheres Using Multiple-Energy Asaxs" @default.
- W2079213532 doi "https://doi.org/10.1016/j.bpj.2010.12.626" @default.
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