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- W2079222499 abstract "A sensitive and specific liquid chromatography electrospray ionization mass spectrometry (LC–ESI-MS) method has been developed and validated for the identification and quantification of lipoic acid (LA) in human plasma. LA and the internal standard, naproxen, were extracted from a 500 μl plasma sample by one-step deproteination using acetonitrile. Chromatographic separation was performed on a Zorbax SB-C18 Column (100 mm × 3.0 mm i.d. with 3.5 μm particle size) with the mobile phase consisting of acetonitrile and 0.1% acetic acid (pH 4, adjusted with ammonia solution) (65:35, v/v), and the flow rate was set at 0.3 ml/min. Detection was performed on a single quadrupole mass spectrometer by selected ion monitoring (SIM) mode via electrospray ionization (ESI) source. The method was linear over the concentration range of 5–10,000 ng/ml for LA. The intra- and inter-day precisions were less than 7% and accuracy ranged from −7.87 to 9.74% at the LA concentrations tested. The present method provides a relatively simple and sensitive assay with short turn-around time. The method has been successfully applied to a clinical pharmacokinetic study of LA in 10 healthy subjects." @default.
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- W2079222499 date "2005-09-25" @default.
- W2079222499 modified "2023-09-28" @default.
- W2079222499 title "Quantification of lipoic acid in plasma by high-performance liquid chromatography–electrospray ionization mass spectrometry" @default.
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- W2079222499 doi "https://doi.org/10.1016/j.jchromb.2005.07.038" @default.
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