FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079244115> ?p ?o ?g. }

• W2079244115 endingPage "99" @default.
• W2079244115 startingPage "91" @default.
• W2079244115 abstract "The method used by YAGYU et al. for the subtype-specific polymerase chain reaction (PCR) amplification of the gp41 transmembrane region of the human immunodeficiency virus type-1 (HIV-1) env gene, was tested. HIV-1 proviral DNA from 100 infected individuals in Itajaí, South Brazil was used to analyze this method. Seventy individuals were determined according to this method as having PCR products at the expected size for subtypes B, C, D and F. Of these individuals, 26 (37.1%) were observed as having the expected amplification for subtype C, and 42 (60%) were observed as having the expected products for subtypes B and D. Of the subtype B and D amplicons, 16 (22.9%) were classified as subtype D, and 26 (37.1%) were classified as subtype B. Two individuals (2.9%) had amplicons that were observed after subtype F-specific amplification was performed. Sequencing and comparing the patient sequences to reference sequences confirmed the classification of sequences of subtypes C and B. However, sequences that were falsely determined as being D and F in the PCR assay were determined as being subtypes C and B, respectively, by sequence analysis. For those individuals from whom no amplified products were obtained, a low viral load that was indicated in their patient history may explain the difficulty in subtyping by PCR methods. This issue was demonstrated by the results of ANOVA when testing the effect of viral load on the success of PCR amplification. The alignment of the obtained sequences with HIV-1 reference sequences demonstrated that there is high intra-subtype diversity. This indicates that the subtype-specific primer binding sites were not conserved or representative of the subtypes that are observed in the Brazilian populations, and that they did not allow the correct classification of HIV-1 subtypes. Therefore, the proposed method by YAGYU et al. is not applicable for the classification of Brazilian HIV-1 subtypes." @default.
• W2079244115 created "2016-06-24" @default.
• W2079244115 creator A5010590300 @default.
• W2079244115 creator A5031737527 @default.
• W2079244115 creator A5034490768 @default.
• W2079244115 creator A5068644191 @default.
• W2079244115 creator A5083229369 @default.
• W2079244115 date "2013-04-01" @default.
• W2079244115 modified "2023-10-01" @default.
• W2079244115 title "TESTING A SUBTYPE-SPECIFIC GP41 AMPLIFICATION METHOD FOR GENOTYPING INDIVIDUALS INFECTED BY HUMAN IMMUNODEFICIENCY VIRUS TYPE-1 IN THE BRAZILIAN POPULATION OF ITAJAÍ, SOUTH BRAZIL" @default.
• W2079244115 cites W1602599521 @default.
• W2079244115 cites W1966185170 @default.
• W2079244115 cites W1978977726 @default.
• W2079244115 cites W1986296710 @default.
• W2079244115 cites W2009187050 @default.
• W2079244115 cites W2015237638 @default.
• W2079244115 cites W2025972413 @default.
• W2079244115 cites W2033688534 @default.
• W2079244115 cites W2038583205 @default.
• W2079244115 cites W2045206031 @default.
• W2079244115 cites W2055349723 @default.
• W2079244115 cites W2082042256 @default.
• W2079244115 cites W2085919925 @default.
• W2079244115 cites W2102801339 @default.
• W2079244115 cites W2113105740 @default.
• W2079244115 cites W2126923818 @default.
• W2079244115 cites W2132632499 @default.
• W2079244115 cites W2149060381 @default.
• W2079244115 cites W2150018908 @default.
• W2079244115 cites W2152069819 @default.
• W2079244115 cites W2158432006 @default.
• W2079244115 cites W2158666214 @default.
• W2079244115 cites W2163230275 @default.
• W2079244115 doi "https://doi.org/10.1590/s0036-46652013000200005" @default.
• W2079244115 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23563761" @default.
• W2079244115 hasPublicationYear "2013" @default.
• W2079244115 type Work @default.
• W2079244115 sameAs 2079244115 @default.
• W2079244115 citedByCount "2" @default.
• W2079244115 countsByYear W20792441152017 @default.
• W2079244115 countsByYear W20792441152021 @default.
• W2079244115 crossrefType "journal-article" @default.
• W2079244115 hasAuthorship W2079244115A5010590300 @default.
• W2079244115 hasAuthorship W2079244115A5031737527 @default.
• W2079244115 hasAuthorship W2079244115A5034490768 @default.
• W2079244115 hasAuthorship W2079244115A5068644191 @default.
• W2079244115 hasAuthorship W2079244115A5083229369 @default.
• W2079244115 hasBestOaLocation W20792441151 @default.
• W2079244115 hasConcept C104317684 @default.
• W2079244115 hasConcept C135763542 @default.
• W2079244115 hasConcept C153911025 @default.
• W2079244115 hasConcept C159047783 @default.
• W2079244115 hasConcept C159654299 @default.
• W2079244115 hasConcept C195616568 @default.
• W2079244115 hasConcept C199360897 @default.
• W2079244115 hasConcept C2522874641 @default.
• W2079244115 hasConcept C2776258796 @default.
• W2079244115 hasConcept C2908647359 @default.
• W2079244115 hasConcept C31467283 @default.
• W2079244115 hasConcept C41008148 @default.
• W2079244115 hasConcept C49105822 @default.
• W2079244115 hasConcept C54355233 @default.
• W2079244115 hasConcept C71924100 @default.
• W2079244115 hasConcept C8185291 @default.
• W2079244115 hasConcept C83852419 @default.
• W2079244115 hasConcept C86803240 @default.
• W2079244115 hasConcept C99454951 @default.
• W2079244115 hasConceptScore W2079244115C104317684 @default.
• W2079244115 hasConceptScore W2079244115C135763542 @default.
• W2079244115 hasConceptScore W2079244115C153911025 @default.
• W2079244115 hasConceptScore W2079244115C159047783 @default.
• W2079244115 hasConceptScore W2079244115C159654299 @default.
• W2079244115 hasConceptScore W2079244115C195616568 @default.
• W2079244115 hasConceptScore W2079244115C199360897 @default.
• W2079244115 hasConceptScore W2079244115C2522874641 @default.
• W2079244115 hasConceptScore W2079244115C2776258796 @default.
• W2079244115 hasConceptScore W2079244115C2908647359 @default.
• W2079244115 hasConceptScore W2079244115C31467283 @default.
• W2079244115 hasConceptScore W2079244115C41008148 @default.
• W2079244115 hasConceptScore W2079244115C49105822 @default.
• W2079244115 hasConceptScore W2079244115C54355233 @default.
• W2079244115 hasConceptScore W2079244115C71924100 @default.
• W2079244115 hasConceptScore W2079244115C8185291 @default.
• W2079244115 hasConceptScore W2079244115C83852419 @default.
• W2079244115 hasConceptScore W2079244115C86803240 @default.
• W2079244115 hasConceptScore W2079244115C99454951 @default.
• W2079244115 hasIssue "2" @default.
• W2079244115 hasLocation W20792441151 @default.
• W2079244115 hasLocation W20792441152 @default.
• W2079244115 hasLocation W20792441153 @default.
• W2079244115 hasLocation W20792441154 @default.
• W2079244115 hasLocation W20792441155 @default.
• W2079244115 hasOpenAccess W2079244115 @default.
• W2079244115 hasPrimaryLocation W20792441151 @default.
• W2079244115 hasRelatedWork W1487857142 @default.
• W2079244115 hasRelatedWork W1996350207 @default.
• W2079244115 hasRelatedWork W2068209906 @default.
• W2079244115 hasRelatedWork W2079244115 @default.

• next