FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079244641> ?p ?o ?g. }

• W2079244641 endingPage "14876" @default.
• W2079244641 startingPage "14871" @default.
• W2079244641 abstract "The Arp2/3 complex, a seven-subunit protein complex containing two actin-related proteins, Arp2 and Arp3, initiates formation of actin filament networks in response to intracellular signals. The molecular mechanism of filament nucleation, however, is not well understood. Arp2 and Arp3 are predicted to bind ATP via a highly conserved nucleotide-binding domain found in all members of the actin superfamily and to form a heterodimer than mimics a conventional actin dimer. We show here that adenosine nucleotides bind with micromolar affinity to both Arp2 and Arp3 and that hydrolyzable ATP is required for actin nucleation activity. Binding of N-WASP WA increases the affinity of both Arp2 and Arp3 for ATP but does not alter the stoichiometry of nucleotides bound in the presence of saturating concentrations of ATP. The Arp2/3 complex bound to ADP or the nonhydrolyzable ATP analogue AMP-PNP cannot nucleate actin filaments, but addition of the phosphate analogue BeF 3 partially restores activity to the ADP–Arp2/3 complex. Bound nucleotide also regulates the affinity of the Arp2/3 complex for its upstream activators N-WASP and ActA. We propose that the active nucleating form of the Arp2/3 complex is the ADP-P i intermediate in the ATPase cycle and that the ATPase activity of the Arp2/3 complex controls both nucleation of new filaments and release of the Arp2/3 complex from membrane-associated activators." @default.
• W2079244641 created "2016-06-24" @default.
• W2079244641 creator A5001893117 @default.
• W2079244641 creator A5027559931 @default.
• W2079244641 creator A5045251012 @default.
• W2079244641 date "2001-12-18" @default.
• W2079244641 modified "2023-10-05" @default.
• W2079244641 title "Arp2/3 complex requires hydrolyzable ATP for nucleation of new actin filaments" @default.
• W2079244641 cites W1487994810 @default.
• W2079244641 cites W1508246694 @default.
• W2079244641 cites W1524475817 @default.
• W2079244641 cites W1539622248 @default.
• W2079244641 cites W1576952319 @default.
• W2079244641 cites W1582738866 @default.
• W2079244641 cites W1595181072 @default.
• W2079244641 cites W1635520329 @default.
• W2079244641 cites W1967955638 @default.
• W2079244641 cites W1971256750 @default.
• W2079244641 cites W2004396935 @default.
• W2079244641 cites W2007660746 @default.
• W2079244641 cites W2010147661 @default.
• W2079244641 cites W2028905487 @default.
• W2079244641 cites W2032049213 @default.
• W2079244641 cites W2032536046 @default.
• W2079244641 cites W2033735656 @default.
• W2079244641 cites W2038136966 @default.
• W2079244641 cites W2039723960 @default.
• W2079244641 cites W2045621376 @default.
• W2079244641 cites W2048987629 @default.
• W2079244641 cites W2049811270 @default.
• W2079244641 cites W2049811440 @default.
• W2079244641 cites W2055801327 @default.
• W2079244641 cites W2061207707 @default.
• W2079244641 cites W2072837740 @default.
• W2079244641 cites W2079099921 @default.
• W2079244641 cites W2080900809 @default.
• W2079244641 cites W2088899830 @default.
• W2079244641 cites W2093226717 @default.
• W2079244641 cites W2099299706 @default.
• W2079244641 cites W2101873410 @default.
• W2079244641 cites W2105789974 @default.
• W2079244641 cites W2107625881 @default.
• W2079244641 cites W2122782850 @default.
• W2079244641 cites W2155653297 @default.
• W2079244641 cites W2160459388 @default.
• W2079244641 cites W2169045417 @default.
• W2079244641 cites W72796490 @default.
• W2079244641 cites W965034824 @default.
• W2079244641 doi "https://doi.org/10.1073/pnas.261419298" @default.
• W2079244641 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/64951" @default.
• W2079244641 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11752435" @default.
• W2079244641 hasPublicationYear "2001" @default.
• W2079244641 type Work @default.
• W2079244641 sameAs 2079244641 @default.
• W2079244641 citedByCount "91" @default.
• W2079244641 countsByYear W20792446412012 @default.
• W2079244641 countsByYear W20792446412013 @default.
• W2079244641 countsByYear W20792446412014 @default.
• W2079244641 countsByYear W20792446412015 @default.
• W2079244641 countsByYear W20792446412016 @default.
• W2079244641 countsByYear W20792446412017 @default.
• W2079244641 countsByYear W20792446412018 @default.
• W2079244641 countsByYear W20792446412020 @default.
• W2079244641 countsByYear W20792446412022 @default.
• W2079244641 countsByYear W20792446412023 @default.
• W2079244641 crossrefType "journal-article" @default.
• W2079244641 hasAuthorship W2079244641A5001893117 @default.
• W2079244641 hasAuthorship W2079244641A5027559931 @default.
• W2079244641 hasAuthorship W2079244641A5045251012 @default.
• W2079244641 hasBestOaLocation W20792446412 @default.
• W2079244641 hasConcept C104317684 @default.
• W2079244641 hasConcept C12554922 @default.
• W2079244641 hasConcept C125705527 @default.
• W2079244641 hasConcept C130861313 @default.
• W2079244641 hasConcept C14228908 @default.
• W2079244641 hasConcept C142669718 @default.
• W2079244641 hasConcept C1491633281 @default.
• W2079244641 hasConcept C189040839 @default.
• W2079244641 hasConcept C2993400109 @default.
• W2079244641 hasConcept C3620293 @default.
• W2079244641 hasConcept C512185932 @default.
• W2079244641 hasConcept C55493867 @default.
• W2079244641 hasConcept C59999672 @default.
• W2079244641 hasConcept C86803240 @default.
• W2079244641 hasConcept C95444343 @default.
• W2079244641 hasConceptScore W2079244641C104317684 @default.
• W2079244641 hasConceptScore W2079244641C12554922 @default.
• W2079244641 hasConceptScore W2079244641C125705527 @default.
• W2079244641 hasConceptScore W2079244641C130861313 @default.
• W2079244641 hasConceptScore W2079244641C14228908 @default.
• W2079244641 hasConceptScore W2079244641C142669718 @default.
• W2079244641 hasConceptScore W2079244641C1491633281 @default.
• W2079244641 hasConceptScore W2079244641C189040839 @default.
• W2079244641 hasConceptScore W2079244641C2993400109 @default.
• W2079244641 hasConceptScore W2079244641C3620293 @default.
• W2079244641 hasConceptScore W2079244641C512185932 @default.
• W2079244641 hasConceptScore W2079244641C55493867 @default.
• W2079244641 hasConceptScore W2079244641C59999672 @default.

• next