Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079248135> ?p ?o ?g. }
Showing items 1 to 90 of
90
with 100 items per page.
- W2079248135 endingPage "BCI.S21580" @default.
- W2079248135 startingPage "BCI.S21580" @default.
- W2079248135 abstract "Cholest-4-en-3-one has positive uses against obesity, liver disease, and keratinization. It can be applied in the synthesis of steroid drugs as well. Most related studies are focused on preparation of cholest-4-en-3-one by using whole cells as catalysts, but production of high-quality cholest-4-en-3-one directly from cholesterol oxidase (COD) using an aqueous/organic two-phase system has been rarely explored. This study set up an enzymatic reaction system to produce cholest-4-en-3-one. We developed and optimized the enzymatic reaction system using COD from COX5-6 (a strain of Rhodococcus) instead of whole-cell biocatalyst. This not only simplifies and accelerates the production but also benefits the subsequent separation and purification process. Through extraction, washing, evaporation, column chromatography, and recrystallization, we got cholest-4-en-3-one with purity of 99.78%, which was identified by nuclear magnetic resonance, mass spectroscopy, and infrared spectroscopy. In addition, this optimized process of cholest-4-en-3-one production and purification can be easily scaled up for industrial production, which can largely decrease the cost and guarantee the purity of the product." @default.
- W2079248135 created "2016-06-24" @default.
- W2079248135 creator A5018716772 @default.
- W2079248135 creator A5021947042 @default.
- W2079248135 creator A5086326013 @default.
- W2079248135 creator A5087998825 @default.
- W2079248135 date "2015-01-01" @default.
- W2079248135 modified "2023-10-16" @default.
- W2079248135 title "Production, Purification, and Identification of Cholest-4-en-3-one Produced by Cholesterol Oxidase from <i>Rhodococcus</i> sp. in Aqueous/Organic Biphasic System" @default.
- W2079248135 cites W1580558317 @default.
- W2079248135 cites W1789017925 @default.
- W2079248135 cites W1946263363 @default.
- W2079248135 cites W1969850018 @default.
- W2079248135 cites W1978565315 @default.
- W2079248135 cites W1995556818 @default.
- W2079248135 cites W2003912483 @default.
- W2079248135 cites W2007847598 @default.
- W2079248135 cites W2009537439 @default.
- W2079248135 cites W2012188810 @default.
- W2079248135 cites W2017993956 @default.
- W2079248135 cites W2027305286 @default.
- W2079248135 cites W2043649713 @default.
- W2079248135 cites W2046914391 @default.
- W2079248135 cites W2049920069 @default.
- W2079248135 cites W2049922840 @default.
- W2079248135 cites W2065291309 @default.
- W2079248135 cites W2065991910 @default.
- W2079248135 cites W2078221716 @default.
- W2079248135 cites W2117783158 @default.
- W2079248135 cites W2139071359 @default.
- W2079248135 cites W2149573662 @default.
- W2079248135 doi "https://doi.org/10.4137/bci.s21580" @default.
- W2079248135 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4334050" @default.
- W2079248135 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25733914" @default.
- W2079248135 hasPublicationYear "2015" @default.
- W2079248135 type Work @default.
- W2079248135 sameAs 2079248135 @default.
- W2079248135 citedByCount "17" @default.
- W2079248135 countsByYear W20792481352015 @default.
- W2079248135 countsByYear W20792481352016 @default.
- W2079248135 countsByYear W20792481352017 @default.
- W2079248135 countsByYear W20792481352018 @default.
- W2079248135 countsByYear W20792481352019 @default.
- W2079248135 countsByYear W20792481352022 @default.
- W2079248135 countsByYear W20792481352023 @default.
- W2079248135 crossrefType "journal-article" @default.
- W2079248135 hasAuthorship W2079248135A5018716772 @default.
- W2079248135 hasAuthorship W2079248135A5021947042 @default.
- W2079248135 hasAuthorship W2079248135A5086326013 @default.
- W2079248135 hasAuthorship W2079248135A5087998825 @default.
- W2079248135 hasBestOaLocation W20792481351 @default.
- W2079248135 hasConcept C178790620 @default.
- W2079248135 hasConcept C181199279 @default.
- W2079248135 hasConcept C184651966 @default.
- W2079248135 hasConcept C185592680 @default.
- W2079248135 hasConcept C2779387492 @default.
- W2079248135 hasConcept C43617362 @default.
- W2079248135 hasConcept C4725764 @default.
- W2079248135 hasConcept C55493867 @default.
- W2079248135 hasConceptScore W2079248135C178790620 @default.
- W2079248135 hasConceptScore W2079248135C181199279 @default.
- W2079248135 hasConceptScore W2079248135C184651966 @default.
- W2079248135 hasConceptScore W2079248135C185592680 @default.
- W2079248135 hasConceptScore W2079248135C2779387492 @default.
- W2079248135 hasConceptScore W2079248135C43617362 @default.
- W2079248135 hasConceptScore W2079248135C4725764 @default.
- W2079248135 hasConceptScore W2079248135C55493867 @default.
- W2079248135 hasLocation W20792481351 @default.
- W2079248135 hasLocation W20792481352 @default.
- W2079248135 hasLocation W20792481353 @default.
- W2079248135 hasLocation W20792481354 @default.
- W2079248135 hasOpenAccess W2079248135 @default.
- W2079248135 hasPrimaryLocation W20792481351 @default.
- W2079248135 hasRelatedWork W1976952012 @default.
- W2079248135 hasRelatedWork W1986558458 @default.
- W2079248135 hasRelatedWork W2073846634 @default.
- W2079248135 hasRelatedWork W2135491076 @default.
- W2079248135 hasRelatedWork W2784534159 @default.
- W2079248135 hasRelatedWork W2949080510 @default.
- W2079248135 hasRelatedWork W2951261188 @default.
- W2079248135 hasRelatedWork W2951314393 @default.
- W2079248135 hasRelatedWork W2951669782 @default.
- W2079248135 hasRelatedWork W2953332163 @default.
- W2079248135 hasVolume "8s1" @default.
- W2079248135 isParatext "false" @default.
- W2079248135 isRetracted "false" @default.
- W2079248135 magId "2079248135" @default.
- W2079248135 workType "article" @default.