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- W2079249282 abstract "Factor V Leiden (FVL) and prothrombin gene mutation may be associated with several complications in pregnancy. There are no published data on prevalence of these mutations in pregnant women in the United Kingdom. This study set out to determine their prevalence in an unselected pregnant population. DNA was extracted from venous blood by a rapid method using proteinase K. Polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) reaction was designed in a novel unpublished technique with mutagenic primers. The system eliminates the false-positive FVL G1691A results caused by rare confounding mutations adjacent to restriction endoclease recognition sites. Following overnight digestion of PCR product by restriction endonucleases (HindIII for prothrombin mutation and BSeM1 for FVL) mutations were detected after polyacrylamide gel vertical electrophoresis. This method is quality controlled by the National External Quality Control Assurance Scheme (NEQAS) from Sheffield. Following electropho..." @default.
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- W2079249282 date "2003-01-01" @default.
- W2079249282 modified "2023-10-17" @default.
- W2079249282 title "Prevalence of factor V Leiden and prothrombin G20210A mutation in 1200 unselected Pregnant Population" @default.
- W2079249282 doi "https://doi.org/10.1080/718591705" @default.
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