Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079249500> ?p ?o ?g. }
- W2079249500 endingPage "3494" @default.
- W2079249500 startingPage "3479" @default.
- W2079249500 abstract "Plasma membrane Intrinsic Proteins (PIPs), a subfamily of aquaporins, are ubiquitous membrane channel proteins that play a crucial role in water uptake in plants. The use of high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) analysis of peptides has previously shown to be a valuable tool to differentiate among PIP homologues sharing a high sequence homology and also to characterize their post-translational modifications (PTMs). The recent introduction of mass spectrometers able to measure peptide mass with high mass accuracy, together with new alternative ways of peptide fragmentation allows the identification and characterization of proteins from nonsequenced organisms, such as broccoli. In this study, we combined three endoproteases (trypsin, Glu-C and Lys-C) with HPLC-MS/MS analysis and two types of peptide fragmentations, CID (collision induced dissociation) and HCD (higher-energy C-trap dissociation), to identify PIP isoforms and PTMs from broccoli roots. After de novo sequencing analysis, eight peptides showing homology to Arabidopsis thaliana PIPs were identified. Although Arabidopsis nomenclature of PIP isoforms has not been defined for broccoli, our results agree with the occurrence of seven AtPIP isoforms (PIP 1;1, PIP 1;2, PIP 1;3 and PIP2;2, PIP 2;3, PIP2;1 and PIP2;7) in broccoli roots, as compared to the plant model A. thaliana. To our knowledge, these results represent the deepest characterization of the PIPs isolated from the roots of broccoli, a crop with increasing agronomical interest." @default.
- W2079249500 created "2016-06-24" @default.
- W2079249500 creator A5034563256 @default.
- W2079249500 creator A5038340407 @default.
- W2079249500 creator A5041751586 @default.
- W2079249500 creator A5042331837 @default.
- W2079249500 creator A5048296305 @default.
- W2079249500 creator A5053687498 @default.
- W2079249500 date "2010-05-28" @default.
- W2079249500 modified "2023-10-14" @default.
- W2079249500 title "Analysis of Root Plasma Membrane Aquaporins from Brassica oleracea: Post-Translational Modifications, de novo Sequencing and Detection of Isoforms by High Resolution Mass Spectrometry" @default.
- W2079249500 cites W1007666405 @default.
- W2079249500 cites W104513617 @default.
- W2079249500 cites W111388303 @default.
- W2079249500 cites W1501694644 @default.
- W2079249500 cites W1512016817 @default.
- W2079249500 cites W1516633194 @default.
- W2079249500 cites W1567378182 @default.
- W2079249500 cites W1918260619 @default.
- W2079249500 cites W1947778485 @default.
- W2079249500 cites W1967790997 @default.
- W2079249500 cites W1971579210 @default.
- W2079249500 cites W1975304761 @default.
- W2079249500 cites W1976001215 @default.
- W2079249500 cites W1987371378 @default.
- W2079249500 cites W1993727030 @default.
- W2079249500 cites W1993918575 @default.
- W2079249500 cites W1996810566 @default.
- W2079249500 cites W1997766826 @default.
- W2079249500 cites W2000760680 @default.
- W2079249500 cites W2002721919 @default.
- W2079249500 cites W2007494100 @default.
- W2079249500 cites W2010182284 @default.
- W2079249500 cites W2015415318 @default.
- W2079249500 cites W2018641191 @default.
- W2079249500 cites W2021829113 @default.
- W2079249500 cites W2026465178 @default.
- W2079249500 cites W2027223134 @default.
- W2079249500 cites W2032363973 @default.
- W2079249500 cites W2033372463 @default.
- W2079249500 cites W2033889320 @default.
- W2079249500 cites W2038423851 @default.
- W2079249500 cites W2042044881 @default.
- W2079249500 cites W2042521088 @default.
- W2079249500 cites W2045247705 @default.
- W2079249500 cites W2052633986 @default.
- W2079249500 cites W2055738510 @default.
- W2079249500 cites W2059919779 @default.
- W2079249500 cites W2066525632 @default.
- W2079249500 cites W2068481474 @default.
- W2079249500 cites W2078594804 @default.
- W2079249500 cites W2078598194 @default.
- W2079249500 cites W2078936885 @default.
- W2079249500 cites W2079725118 @default.
- W2079249500 cites W2081522534 @default.
- W2079249500 cites W2084304818 @default.
- W2079249500 cites W2085217795 @default.
- W2079249500 cites W2086747277 @default.
- W2079249500 cites W2089620206 @default.
- W2079249500 cites W2090082061 @default.
- W2079249500 cites W2090182712 @default.
- W2079249500 cites W2090379283 @default.
- W2079249500 cites W2093189530 @default.
- W2079249500 cites W2093931104 @default.
- W2079249500 cites W2095962304 @default.
- W2079249500 cites W2099255819 @default.
- W2079249500 cites W2101108802 @default.
- W2079249500 cites W2105525923 @default.
- W2079249500 cites W2106882534 @default.
- W2079249500 cites W2111419599 @default.
- W2079249500 cites W2112713465 @default.
- W2079249500 cites W2115422240 @default.
- W2079249500 cites W2124482320 @default.
- W2079249500 cites W2135080621 @default.
- W2079249500 cites W2140674348 @default.
- W2079249500 cites W2140985419 @default.
- W2079249500 cites W2141207650 @default.
- W2079249500 cites W2147032429 @default.
- W2079249500 cites W2152770371 @default.
- W2079249500 cites W2153777219 @default.
- W2079249500 cites W2154365500 @default.
- W2079249500 cites W2155976537 @default.
- W2079249500 cites W2158714788 @default.
- W2079249500 cites W2158897796 @default.
- W2079249500 cites W2163153928 @default.
- W2079249500 cites W2163423253 @default.
- W2079249500 cites W2164234055 @default.
- W2079249500 cites W2167057772 @default.
- W2079249500 cites W2171320885 @default.
- W2079249500 cites W25917353 @default.
- W2079249500 cites W2081238954 @default.
- W2079249500 doi "https://doi.org/10.1021/pr901150g" @default.
- W2079249500 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/20462273" @default.
- W2079249500 hasPublicationYear "2010" @default.
- W2079249500 type Work @default.
- W2079249500 sameAs 2079249500 @default.
- W2079249500 citedByCount "25" @default.
- W2079249500 countsByYear W20792495002013 @default.