Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079254361> ?p ?o ?g. }
- W2079254361 endingPage "e60072" @default.
- W2079254361 startingPage "e60072" @default.
- W2079254361 abstract "Cyclohexylamine oxidase (CHAO) is a flavoprotein first described in Brevibacterium oxydans strain IH-35A that carries out the initial step of the degradation of the industrial chemical cyclohexylamine to cyclohexanone. We have cloned and expressed in Escherichia coli the CHAO-encoding gene (chaA) from B. oxydans, purified CHAO and determined the structures of both the holoenzyme form of the enzyme and a product complex with cyclohexanone. CHAO is a 50 kDa monomer with a PHBH fold topology. It belongs to the flavin monooxygenase family of enzymes and exhibits high substrate specificity for alicyclic amines and sec-alkylamines. The overall structure is similar to that of other members of the flavin monooxygenase family, but lacks either of the C- or N-terminal extensions observed in these enzymes. Active site features of the flavin monooxygenase family are conserved in CHAO, including the characteristic aromatic cage. Differences in the orientations of residues of the CHAO aromatic cage result in a substrate-binding site that is more open than those of its structural relatives. Since CHAO has a buried hydrophobic active site with no obvious route for substrates and products, a random acceleration molecular dynamics simulation has been used to identify a potential egress route. The path identified includes an intermediate cavity and requires transient conformation changes in a shielding loop and a residue at the border of the substrate-binding cavity. These results provide a foundation for further studies with CHAO aimed at identifying features determining substrate specificity and for developing the biocatalytic potential of this enzyme." @default.
- W2079254361 created "2016-06-24" @default.
- W2079254361 creator A5014016591 @default.
- W2079254361 creator A5014609170 @default.
- W2079254361 creator A5036164858 @default.
- W2079254361 creator A5037520740 @default.
- W2079254361 creator A5049742551 @default.
- W2079254361 creator A5066465640 @default.
- W2079254361 creator A5071153074 @default.
- W2079254361 creator A5079673500 @default.
- W2079254361 date "2013-03-26" @default.
- W2079254361 modified "2023-10-10" @default.
- W2079254361 title "Structural Analysis of a Novel Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A" @default.
- W2079254361 cites W124890769 @default.
- W2079254361 cites W1539796472 @default.
- W2079254361 cites W1779976831 @default.
- W2079254361 cites W1960594152 @default.
- W2079254361 cites W1967737979 @default.
- W2079254361 cites W1986717702 @default.
- W2079254361 cites W1994286350 @default.
- W2079254361 cites W1995017064 @default.
- W2079254361 cites W1997866241 @default.
- W2079254361 cites W2006911538 @default.
- W2079254361 cites W2007487695 @default.
- W2079254361 cites W2012516949 @default.
- W2079254361 cites W2013640944 @default.
- W2079254361 cites W2020796834 @default.
- W2079254361 cites W2021265316 @default.
- W2079254361 cites W2029545844 @default.
- W2079254361 cites W2030356351 @default.
- W2079254361 cites W2033015524 @default.
- W2079254361 cites W2033330355 @default.
- W2079254361 cites W2045226520 @default.
- W2079254361 cites W2046204255 @default.
- W2079254361 cites W2084071209 @default.
- W2079254361 cites W2088286639 @default.
- W2079254361 cites W2091392856 @default.
- W2079254361 cites W2098840696 @default.
- W2079254361 cites W2126008297 @default.
- W2079254361 cites W2138825634 @default.
- W2079254361 cites W2140545914 @default.
- W2079254361 cites W2150981663 @default.
- W2079254361 cites W2152382512 @default.
- W2079254361 cites W2564088543 @default.
- W2079254361 cites W4293247451 @default.
- W2079254361 cites W4300880735 @default.
- W2079254361 doi "https://doi.org/10.1371/journal.pone.0060072" @default.
- W2079254361 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3608611" @default.
- W2079254361 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23555888" @default.
- W2079254361 hasPublicationYear "2013" @default.
- W2079254361 type Work @default.
- W2079254361 sameAs 2079254361 @default.
- W2079254361 citedByCount "22" @default.
- W2079254361 countsByYear W20792543612013 @default.
- W2079254361 countsByYear W20792543612014 @default.
- W2079254361 countsByYear W20792543612015 @default.
- W2079254361 countsByYear W20792543612016 @default.
- W2079254361 countsByYear W20792543612017 @default.
- W2079254361 countsByYear W20792543612018 @default.
- W2079254361 countsByYear W20792543612020 @default.
- W2079254361 countsByYear W20792543612021 @default.
- W2079254361 countsByYear W20792543612022 @default.
- W2079254361 crossrefType "journal-article" @default.
- W2079254361 hasAuthorship W2079254361A5014016591 @default.
- W2079254361 hasAuthorship W2079254361A5014609170 @default.
- W2079254361 hasAuthorship W2079254361A5036164858 @default.
- W2079254361 hasAuthorship W2079254361A5037520740 @default.
- W2079254361 hasAuthorship W2079254361A5049742551 @default.
- W2079254361 hasAuthorship W2079254361A5066465640 @default.
- W2079254361 hasAuthorship W2079254361A5071153074 @default.
- W2079254361 hasAuthorship W2079254361A5079673500 @default.
- W2079254361 hasBestOaLocation W20792543611 @default.
- W2079254361 hasConcept C13002179 @default.
- W2079254361 hasConcept C178790620 @default.
- W2079254361 hasConcept C181199279 @default.
- W2079254361 hasConcept C185592680 @default.
- W2079254361 hasConcept C18903297 @default.
- W2079254361 hasConcept C2777289219 @default.
- W2079254361 hasConcept C2777346864 @default.
- W2079254361 hasConcept C33093398 @default.
- W2079254361 hasConcept C41183919 @default.
- W2079254361 hasConcept C526171541 @default.
- W2079254361 hasConcept C55493867 @default.
- W2079254361 hasConcept C71240020 @default.
- W2079254361 hasConcept C71995715 @default.
- W2079254361 hasConcept C86803240 @default.
- W2079254361 hasConceptScore W2079254361C13002179 @default.
- W2079254361 hasConceptScore W2079254361C178790620 @default.
- W2079254361 hasConceptScore W2079254361C181199279 @default.
- W2079254361 hasConceptScore W2079254361C185592680 @default.
- W2079254361 hasConceptScore W2079254361C18903297 @default.
- W2079254361 hasConceptScore W2079254361C2777289219 @default.
- W2079254361 hasConceptScore W2079254361C2777346864 @default.
- W2079254361 hasConceptScore W2079254361C33093398 @default.
- W2079254361 hasConceptScore W2079254361C41183919 @default.
- W2079254361 hasConceptScore W2079254361C526171541 @default.
- W2079254361 hasConceptScore W2079254361C55493867 @default.
- W2079254361 hasConceptScore W2079254361C71240020 @default.