Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079254451> ?p ?o ?g. }
Showing items 1 to 94 of
94
with 100 items per page.
- W2079254451 endingPage "739" @default.
- W2079254451 startingPage "735" @default.
- W2079254451 abstract "Abstract A new phenylalanine ammonia-lyase (PAL) was purified from leaves of Ocimum basilicum L. (chemotype, methyl cinnamate). Separation techniques applied icluded anion exchange chromatography and preparative electroelution from a non-denaturing polyacrylamide gel. A 180-fold purification was obtained. The native enzyme was a homotetramer of Mr 152 000–153 000; the intact subunit Mr was ca 38 000. The enzyme catalysed the conversion of l -phenylalanine -d 8 into trans-cinnamic acid-d7, as determined by GC-mass spectral analysis of silylated reaction products. The purified native enzyme had Km and Vmax values of 329 μM and 11.43 μmol min−1 mg−1 protein, respectively, for l -phenylalanine and was competitively inhibited by 2-aminoindan-2-phosphonic acid, trans-cinnamic acid and trans-methyl cinnamate with Ki values of 19 nM and 57 and 130 μM, respectively. Comparing the Ki values between trans-cinnamic acid and trans-methyl cinnamate for l -phenylalanine indicated that the regulation of PAL is not only related to the mechanism of feedback inhibition in the biosynthesis of trans-methyl cinnamate." @default.
- W2079254451 created "2016-06-24" @default.
- W2079254451 creator A5010909508 @default.
- W2079254451 creator A5016187030 @default.
- W2079254451 creator A5058457549 @default.
- W2079254451 creator A5065742203 @default.
- W2079254451 date "1996-11-01" @default.
- W2079254451 modified "2023-10-18" @default.
- W2079254451 title "Purification and characterization of a phenylalanine ammonia-lyase from Ocimum basilicum" @default.
- W2079254451 cites W1549493910 @default.
- W2079254451 cites W1997817543 @default.
- W2079254451 cites W2022239748 @default.
- W2079254451 cites W2025558389 @default.
- W2079254451 cites W2036155160 @default.
- W2079254451 cites W2038345597 @default.
- W2079254451 cites W2045742306 @default.
- W2079254451 cites W2050077740 @default.
- W2079254451 cites W2056374484 @default.
- W2079254451 cites W2092055824 @default.
- W2079254451 cites W2100837269 @default.
- W2079254451 cites W2146848185 @default.
- W2079254451 cites W2949975424 @default.
- W2079254451 cites W4293247451 @default.
- W2079254451 doi "https://doi.org/10.1016/0031-9422(96)00168-9" @default.
- W2079254451 hasPublicationYear "1996" @default.
- W2079254451 type Work @default.
- W2079254451 sameAs 2079254451 @default.
- W2079254451 citedByCount "20" @default.
- W2079254451 countsByYear W20792544512012 @default.
- W2079254451 countsByYear W20792544512014 @default.
- W2079254451 countsByYear W20792544512015 @default.
- W2079254451 countsByYear W20792544512016 @default.
- W2079254451 countsByYear W20792544512020 @default.
- W2079254451 countsByYear W20792544512022 @default.
- W2079254451 crossrefType "journal-article" @default.
- W2079254451 hasAuthorship W2079254451A5010909508 @default.
- W2079254451 hasAuthorship W2079254451A5016187030 @default.
- W2079254451 hasAuthorship W2079254451A5058457549 @default.
- W2079254451 hasAuthorship W2079254451A5065742203 @default.
- W2079254451 hasConcept C171250308 @default.
- W2079254451 hasConcept C178790620 @default.
- W2079254451 hasConcept C181199279 @default.
- W2079254451 hasConcept C185592680 @default.
- W2079254451 hasConcept C192562407 @default.
- W2079254451 hasConcept C2776382133 @default.
- W2079254451 hasConcept C2777269803 @default.
- W2079254451 hasConcept C2777431362 @default.
- W2079254451 hasConcept C2777612184 @default.
- W2079254451 hasConcept C2778112864 @default.
- W2079254451 hasConcept C2779342943 @default.
- W2079254451 hasConcept C2780841128 @default.
- W2079254451 hasConcept C515207424 @default.
- W2079254451 hasConcept C55493867 @default.
- W2079254451 hasConcept C59822182 @default.
- W2079254451 hasConcept C71240020 @default.
- W2079254451 hasConcept C86803240 @default.
- W2079254451 hasConceptScore W2079254451C171250308 @default.
- W2079254451 hasConceptScore W2079254451C178790620 @default.
- W2079254451 hasConceptScore W2079254451C181199279 @default.
- W2079254451 hasConceptScore W2079254451C185592680 @default.
- W2079254451 hasConceptScore W2079254451C192562407 @default.
- W2079254451 hasConceptScore W2079254451C2776382133 @default.
- W2079254451 hasConceptScore W2079254451C2777269803 @default.
- W2079254451 hasConceptScore W2079254451C2777431362 @default.
- W2079254451 hasConceptScore W2079254451C2777612184 @default.
- W2079254451 hasConceptScore W2079254451C2778112864 @default.
- W2079254451 hasConceptScore W2079254451C2779342943 @default.
- W2079254451 hasConceptScore W2079254451C2780841128 @default.
- W2079254451 hasConceptScore W2079254451C515207424 @default.
- W2079254451 hasConceptScore W2079254451C55493867 @default.
- W2079254451 hasConceptScore W2079254451C59822182 @default.
- W2079254451 hasConceptScore W2079254451C71240020 @default.
- W2079254451 hasConceptScore W2079254451C86803240 @default.
- W2079254451 hasIssue "4" @default.
- W2079254451 hasLocation W20792544511 @default.
- W2079254451 hasOpenAccess W2079254451 @default.
- W2079254451 hasPrimaryLocation W20792544511 @default.
- W2079254451 hasRelatedWork W1966194447 @default.
- W2079254451 hasRelatedWork W1969771289 @default.
- W2079254451 hasRelatedWork W1971805583 @default.
- W2079254451 hasRelatedWork W2050733347 @default.
- W2079254451 hasRelatedWork W2057456090 @default.
- W2079254451 hasRelatedWork W2093901820 @default.
- W2079254451 hasRelatedWork W2288905183 @default.
- W2079254451 hasRelatedWork W2389422110 @default.
- W2079254451 hasRelatedWork W2808318619 @default.
- W2079254451 hasRelatedWork W4252750918 @default.
- W2079254451 hasVolume "43" @default.
- W2079254451 isParatext "false" @default.
- W2079254451 isRetracted "false" @default.
- W2079254451 magId "2079254451" @default.
- W2079254451 workType "article" @default.