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- W2079256278 abstract "It was previously assumed that E·LH 2 ‐AMP was the intermediate complex in the synthesis of Ap 4 A catalyzed by firefly luciferase (EC 1.13.12.7), when luciferin (LH 2 ) was used as cofactor. However, here we show that LH 2 is partly transformed, shortly after the onset of the luciferase reaction, to dehydroluciferin (L) with formation of an E·L‐AMP complex which is the main intermediate for the synthesis of Ap 4 A. Formation of three more derivatives of LH 2 were also observed, related to the production of light by the enzyme. CoA, a known stimulator of light production, inhibits the synthesis of Ap 4 A by reacting with the E·L‐AMP complex and yielding L‐CoA." @default.
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- W2079256278 date "1998-11-06" @default.
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- W2079256278 title "Dehydroluciferyl‐AMP is the main intermediate in the luciferin dependent synthesis of Ap<sub>4</sub>A catalyzed by firefly luciferase" @default.
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- W2079256278 doi "https://doi.org/10.1016/s0014-5793(98)01301-5" @default.
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