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• W2079257324 abstract "Automated protein function annotation is extremely important in computational biology for its low cost. Standard sequence similarity comparison methods for annotation have limited specificity in identifying orthologs and paralogs. Phylogenomic methods are gaining popularity for their role in identifying orthologs and paralogs with the help of evolutionary information and sequence data. Pipelines have been developed for phylogenomic classification of proteins. Two such pipelines are PhyloFacts and PipeAlign. Given a protein of interest, these pipelines identify functional subfamilies for the protein superfamily. Subfamilies hold orthologs and paralogs and can later be used to identify orthologous groups. We evaluate the performance of PipeAlign with respect to both consistency in the generated subfamilies and phylogeny. We use the predefined subfamilies of PhyloFacts as a reference to compare the generated subfamilies of related reference sequences in PipeAlign. In the consistency analysis, we compare the compositions of the generated functional subfamilies with different related reference sequences, and use the predefined PhyloFacts subfamilies for the corresponding sequences as a measure of consistency. In the phylogenetic analysis, we compare the evolutionary distances of the members of the same and different generated subfamilies from PipeAlign." @default.
• W2079257324 created "2016-06-24" @default.
• W2079257324 creator A5018731337 @default.
• W2079257324 creator A5041471776 @default.
• W2079257324 date "2010-05-01" @default.
• W2079257324 modified "2023-09-25" @default.
• W2079257324 title "Issues with the PipeAlign phylogenomics toolkit in identifying protein subfamilies" @default.
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• W2079257324 doi "https://doi.org/10.1109/cibcb.2010.5510344" @default.
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