Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079261085> ?p ?o ?g. }
Showing items 1 to 87 of
87
with 100 items per page.
- W2079261085 endingPage "8051" @default.
- W2079261085 startingPage "8047" @default.
- W2079261085 abstract "We report the development of two dominant-acting genetic markers useful for monitoring gene transfer in mammalian cells that are based on prokaryotic genes encoding key steps in the synthesis of the essential amino acids, tryptophan and histidine. Under appropriate conditions, expression of these genes obviates the nutritional requirements for their respective amino acid products. Expression of the trpB gene of Escherichia coli, which encodes the beta subunit of tryptophan synthase (EC 4.2.1.20), allows mammalian cell survival and multiplication in medium containing indole in place of tryptophan. The hisD gene of Salmonella typhimurium encodes histidinol dehydrogenase (EC 1.1.1.23), which catalyzes the two-step NAD+-dependent oxidation of L-histidinol to L-histidine. In medium lacking histidine and containing histidinol, only mammalian cells expressing the hisD product survive. The selection is a double one in that the provided precursor histidinol is itself toxic to animal cells through its inhibition of histidyl-tRNA synthetase; thus, the dehydrogenase both removes an inhibitor and forms a required end product. Alternatively, the his selection may be carried out under conditions in which the dehydrogenase serves mainly to detoxify histidinol. For either the trp or his selections the substitute nutrient (indole or histidinol) is readily available, inexpensive, stable, permeable to cells, and convertible to the end product in a step controlled by a single gene. Vectors based upon murine retrovirus and papovavirus backbones have been successfully employed for both genes, allowing selection in a range of cell types, including 3T3, CV-1, and HeLa. These dominant selective schemes should provide generally useful and inexpensive alternatives to others currently in use, such as the gpt, neo, hygro, dhfr, and tk selections." @default.
- W2079261085 created "2016-06-24" @default.
- W2079261085 creator A5054504442 @default.
- W2079261085 creator A5066302335 @default.
- W2079261085 date "1988-11-01" @default.
- W2079261085 modified "2023-09-23" @default.
- W2079261085 title "Two dominant-acting selectable markers for gene transfer studies in mammalian cells." @default.
- W2079261085 cites W1558516755 @default.
- W2079261085 cites W1559585838 @default.
- W2079261085 cites W1693784704 @default.
- W2079261085 cites W1913773160 @default.
- W2079261085 cites W1964265039 @default.
- W2079261085 cites W1971686894 @default.
- W2079261085 cites W1980826960 @default.
- W2079261085 cites W2012134426 @default.
- W2079261085 cites W2027411626 @default.
- W2079261085 cites W2032286831 @default.
- W2079261085 cites W2053654911 @default.
- W2079261085 cites W2054335050 @default.
- W2079261085 cites W2056618475 @default.
- W2079261085 cites W2063450941 @default.
- W2079261085 cites W2075936616 @default.
- W2079261085 cites W2076297147 @default.
- W2079261085 cites W2088336419 @default.
- W2079261085 cites W2094657382 @default.
- W2079261085 cites W2113208052 @default.
- W2079261085 cites W2121349955 @default.
- W2079261085 cites W2161573420 @default.
- W2079261085 cites W2343889395 @default.
- W2079261085 cites W70323815 @default.
- W2079261085 doi "https://doi.org/10.1073/pnas.85.21.8047" @default.
- W2079261085 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/282351" @default.
- W2079261085 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3054879" @default.
- W2079261085 hasPublicationYear "1988" @default.
- W2079261085 type Work @default.
- W2079261085 sameAs 2079261085 @default.
- W2079261085 citedByCount "171" @default.
- W2079261085 countsByYear W20792610852012 @default.
- W2079261085 countsByYear W20792610852013 @default.
- W2079261085 countsByYear W20792610852015 @default.
- W2079261085 countsByYear W20792610852016 @default.
- W2079261085 countsByYear W20792610852018 @default.
- W2079261085 countsByYear W20792610852019 @default.
- W2079261085 crossrefType "journal-article" @default.
- W2079261085 hasAuthorship W2079261085A5054504442 @default.
- W2079261085 hasAuthorship W2079261085A5066302335 @default.
- W2079261085 hasBestOaLocation W20792610851 @default.
- W2079261085 hasConcept C104317684 @default.
- W2079261085 hasConcept C150194340 @default.
- W2079261085 hasConcept C153911025 @default.
- W2079261085 hasConcept C195286587 @default.
- W2079261085 hasConcept C2778460671 @default.
- W2079261085 hasConcept C515207424 @default.
- W2079261085 hasConcept C55493867 @default.
- W2079261085 hasConcept C86803240 @default.
- W2079261085 hasConceptScore W2079261085C104317684 @default.
- W2079261085 hasConceptScore W2079261085C150194340 @default.
- W2079261085 hasConceptScore W2079261085C153911025 @default.
- W2079261085 hasConceptScore W2079261085C195286587 @default.
- W2079261085 hasConceptScore W2079261085C2778460671 @default.
- W2079261085 hasConceptScore W2079261085C515207424 @default.
- W2079261085 hasConceptScore W2079261085C55493867 @default.
- W2079261085 hasConceptScore W2079261085C86803240 @default.
- W2079261085 hasIssue "21" @default.
- W2079261085 hasLocation W20792610851 @default.
- W2079261085 hasLocation W20792610852 @default.
- W2079261085 hasLocation W20792610853 @default.
- W2079261085 hasLocation W20792610854 @default.
- W2079261085 hasOpenAccess W2079261085 @default.
- W2079261085 hasPrimaryLocation W20792610851 @default.
- W2079261085 hasRelatedWork W188155302 @default.
- W2079261085 hasRelatedWork W1971645214 @default.
- W2079261085 hasRelatedWork W2009966535 @default.
- W2079261085 hasRelatedWork W2033656695 @default.
- W2079261085 hasRelatedWork W2037995085 @default.
- W2079261085 hasRelatedWork W2088411685 @default.
- W2079261085 hasRelatedWork W2096293222 @default.
- W2079261085 hasRelatedWork W2137360360 @default.
- W2079261085 hasRelatedWork W311012215 @default.
- W2079261085 hasRelatedWork W4309098386 @default.
- W2079261085 hasVolume "85" @default.
- W2079261085 isParatext "false" @default.
- W2079261085 isRetracted "false" @default.
- W2079261085 magId "2079261085" @default.
- W2079261085 workType "article" @default.