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- W2079261489 abstract "Auxotrophic and analogue-resistant mutants were isolated from nitrosoguanidine-treated populations of Methanobacterium thermoautotrophicum strain Marburg. Auxotrophs requiring L-leucine, L-phenyl-alanine, L-tryptophan, adenosine or thiamin were found. An enrichment procedure for auxotrophs based on the preferential killing of growing cells by bacitracin was developed. In simulation experiments the method permitted a 50-fold enrichment of auxotrophs. It proved effective in a mutant hunt leading to a 5% yield of auxotrophs in mutagenized populations subjected to bacitracin enrichment. Mutants resistant to 2-bromoethane sulfonic acid (2-BES), DL-ethionine or pseudomonic acid A were also obtained. Since the ethionine- and 2-BES-resistant mutants exhibited low resistance, impaired plating efficiency and altered cell morphology, they seemed unsuitable for genetic experiments. Pseudomonic acid resistance led to a 50-fold increase in the minimum inhibitory concentration. It was correlated with an isoleucyl-tRNA synthetase activity insensitive to inhitibion by pseudomonic acid. The corresponding enzyme from the wild type was strongly inhibited by pseudomonic acid." @default.
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- W2079261489 date "1986-03-01" @default.
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- W2079261489 title "Mutants of Methanobacterium thermoautotrophicum" @default.
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- W2079261489 doi "https://doi.org/10.1016/s0723-2020(86)80115-1" @default.
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