FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079265304> ?p ?o ?g. }

• W2079265304 endingPage "1788" @default.
• W2079265304 startingPage "1783" @default.
• W2079265304 abstract "o-Phthalaldehyde-β-mercaptoethanol (OPAME) as a fluorogenic reagent has been found wide applications in the detection of amino acids based on its reaction with primary amino groups. In this contribution, we report our new findings concerning the reactions of OPAME with single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA), respectively. It has been found that ssDNA can react with OPAME easily as a result of giving rise to strong fluorescence emissions, while dsDNA, prepared by hybridizing ssDNA with its complementary target prior to the reaction, displays inert chemical activity and gives out weak fluorescence emission. Mechanism investigations have shown that the reaction activity between OPAME and DNA depends on the amino groups that are related to the conformation of uncoiled and exposed extent of DNA structure, and thus the inert chemical activity of dsDNA results from screening of the dsDNA bases in the interior of the double strands. Therefore, we could design a way to detect conformation change of DNA with OPAME and further develop a novel, simple label-free sequence detection method for complementary and single-base mismatched ssDNA in the hybridization of DNA." @default.
• W2079265304 created "2016-06-24" @default.
• W2079265304 creator A5000717105 @default.
• W2079265304 creator A5024931083 @default.
• W2079265304 creator A5038545320 @default.
• W2079265304 creator A5062234550 @default.
• W2079265304 creator A5090173637 @default.
• W2079265304 date "2008-01-24" @default.
• W2079265304 modified "2023-10-16" @default.
• W2079265304 title "Conformational Change Detection of DNA with the Fluorogenic Reagent of <i>o</i>-Phthalaldehyde-β-Mercaptoethanol" @default.
• W2079265304 cites W1968129834 @default.
• W2079265304 cites W1987315229 @default.
• W2079265304 cites W1990025317 @default.
• W2079265304 cites W1990679039 @default.
• W2079265304 cites W1991237916 @default.
• W2079265304 cites W1993629281 @default.
• W2079265304 cites W2008377368 @default.
• W2079265304 cites W2010120270 @default.
• W2079265304 cites W2011954366 @default.
• W2079265304 cites W2015784769 @default.
• W2079265304 cites W2016019039 @default.
• W2079265304 cites W2022615267 @default.
• W2079265304 cites W2027260822 @default.
• W2079265304 cites W2028310736 @default.
• W2079265304 cites W2028410253 @default.
• W2079265304 cites W2031900776 @default.
• W2079265304 cites W2039541561 @default.
• W2079265304 cites W2040842899 @default.
• W2079265304 cites W2054005757 @default.
• W2079265304 cites W2061755273 @default.
• W2079265304 cites W2065459148 @default.
• W2079265304 cites W2065601399 @default.
• W2079265304 cites W2079768304 @default.
• W2079265304 cites W2083424582 @default.
• W2079265304 cites W2088047441 @default.
• W2079265304 cites W2094097453 @default.
• W2079265304 cites W2100269540 @default.
• W2079265304 cites W2101669631 @default.
• W2079265304 cites W2103876253 @default.
• W2079265304 cites W2116678462 @default.
• W2079265304 cites W2120017523 @default.
• W2079265304 cites W2132235135 @default.
• W2079265304 cites W2152116281 @default.
• W2079265304 cites W2159510997 @default.
• W2079265304 cites W2167230638 @default.
• W2079265304 cites W3021276718 @default.
• W2079265304 cites W4237943893 @default.
• W2079265304 doi "https://doi.org/10.1021/jp071601g" @default.
• W2079265304 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18215031" @default.
• W2079265304 hasPublicationYear "2008" @default.
• W2079265304 type Work @default.
• W2079265304 sameAs 2079265304 @default.
• W2079265304 citedByCount "26" @default.
• W2079265304 countsByYear W20792653042012 @default.
• W2079265304 countsByYear W20792653042014 @default.
• W2079265304 countsByYear W20792653042015 @default.
• W2079265304 countsByYear W20792653042016 @default.
• W2079265304 countsByYear W20792653042017 @default.
• W2079265304 countsByYear W20792653042018 @default.
• W2079265304 countsByYear W20792653042019 @default.
• W2079265304 crossrefType "journal-article" @default.
• W2079265304 hasAuthorship W2079265304A5000717105 @default.
• W2079265304 hasAuthorship W2079265304A5024931083 @default.
• W2079265304 hasAuthorship W2079265304A5038545320 @default.
• W2079265304 hasAuthorship W2079265304A5062234550 @default.
• W2079265304 hasAuthorship W2079265304A5090173637 @default.
• W2079265304 hasConcept C104317684 @default.
• W2079265304 hasConcept C121332964 @default.
• W2079265304 hasConcept C162356407 @default.
• W2079265304 hasConcept C178790620 @default.
• W2079265304 hasConcept C185581394 @default.
• W2079265304 hasConcept C185592680 @default.
• W2079265304 hasConcept C21951064 @default.
• W2079265304 hasConcept C2776923573 @default.
• W2079265304 hasConcept C2909762588 @default.
• W2079265304 hasConcept C40875361 @default.
• W2079265304 hasConcept C43617362 @default.
• W2079265304 hasConcept C512185932 @default.
• W2079265304 hasConcept C552990157 @default.
• W2079265304 hasConcept C55493867 @default.
• W2079265304 hasConcept C62520636 @default.
• W2079265304 hasConcept C71240020 @default.
• W2079265304 hasConcept C91881484 @default.
• W2079265304 hasConceptScore W2079265304C104317684 @default.
• W2079265304 hasConceptScore W2079265304C121332964 @default.
• W2079265304 hasConceptScore W2079265304C162356407 @default.
• W2079265304 hasConceptScore W2079265304C178790620 @default.
• W2079265304 hasConceptScore W2079265304C185581394 @default.
• W2079265304 hasConceptScore W2079265304C185592680 @default.
• W2079265304 hasConceptScore W2079265304C21951064 @default.
• W2079265304 hasConceptScore W2079265304C2776923573 @default.
• W2079265304 hasConceptScore W2079265304C2909762588 @default.
• W2079265304 hasConceptScore W2079265304C40875361 @default.
• W2079265304 hasConceptScore W2079265304C43617362 @default.
• W2079265304 hasConceptScore W2079265304C512185932 @default.
• W2079265304 hasConceptScore W2079265304C552990157 @default.
• W2079265304 hasConceptScore W2079265304C55493867 @default.
• W2079265304 hasConceptScore W2079265304C62520636 @default.

• next