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- W2079284003 abstract "A new isotope method of microassay, derivative ratio analysis, has been developed. To the substance to be measured, e.g., testosterone, an unlabeled internal standard with a similar functional group is added. The mixture is acetylated with acetic anhydride-C14 following which the derivatives are separated by gas-liquid chromatography. Detection and measurement of C14 in the effluent of the chromatography is performed either continuously during the analysis or by fractionating the effluent and then assaying each fraction individually. The quantity of unknown is related to the quantity of standard in the same ratio as the radioactivity of the unknown is related to the radioactivity in the standard. This method may be used with a variety of compounds, and may be extended to measurement of nanogram quantities." @default.
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- W2079284003 date "1963-07-01" @default.
- W2079284003 modified "2023-10-13" @default.
- W2079284003 title "Derivative ratio analysis: A new method for measurement of steroids and other compounds with specific functional groups using radioassay by gas-liquid chromatography" @default.
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- W2079284003 doi "https://doi.org/10.1016/0003-2697(63)90005-8" @default.
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