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- W2079285846 abstract "Using a specific antibody (SMI 31), the state of phosphorylation of high and medium molecular weight neurofilaments (NF‐H and NF‐M) was studied in 22 leprous and four nonleprous human peripheral nerves by means of immunohistochemistry, sodium dodecyl sulfate‐poly acrylamide gel electrophoresis (SDS‐PAGE) and Western immunoblot (WB). The results thus obtained were compared with morphological changes in the respective nerves studied through light and electron microscopy. Many of the leprous nerves showing minimal pathology revealed lack of or weak staining with SMI 31, denoting dephosphorylation. Remyelinated fibres stained intensely with SMI 31 antibody. The WB analysis of Triton X‐100 insoluble cytoskeletal preparation showed absence of regular SMI 31 reactive bands corresponding to 200 and 150 kDa molecular weight (NF‐H and NF‐M, respectively) in 10 nerves. Three of the 10 nerves revealed presence of NF protein bands in SDS‐PAGE but not in WB. Presence of additional protein band (following NF‐M) was seen in four nerves. Two nerves revealed NF‐H band but not NF‐M band and one nerve showed trace positivity. In the remaining five nerves presence of all the three NF bands was seen. Thus, 77.3% (17/22) of human leprous nerves studied showed abnormal phosphorylation of NF protein(s). The ultrastructural study showed abnormal compaction and arraying of NF at the periphery of the axons in the fibres with altered axon to myelin thickness ratio (atrophied fibres) as well as at the Schmidt‐Lantermann (S‐L) cleft region. Such NF changes were more pronounced in the severely atrophied axons suggesting a direct correlation. The observed well‐spaced NF in the remyelinated fibres under ultrastructural study was in keeping with both intense SMI 31 staining and presence of NF triplet bands seen in WBs in four of leprous nerves that showed a large number of regenerating fibres suggesting reversal of changes with regeneration. Findings in the present study suggest that atrophy, that is, the reduction in axonal calibre and paranodal demyelination, seen in leprous nerves may result from dephosphorylation of NF‐H and NF‐M proteins." @default.
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- W2079285846 date "2004-07-09" @default.
- W2079285846 modified "2023-10-16" @default.
- W2079285846 title "Alterations in neurofilament protein(s) in human leprous nerves: morphology, immunohistochemistry and Western immunoblot correlative study" @default.
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- W2079285846 cites W1973144980 @default.
- W2079285846 cites W1973766365 @default.
- W2079285846 cites W1980624520 @default.
- W2079285846 cites W1981097683 @default.
- W2079285846 cites W1981380552 @default.
- W2079285846 cites W1982647438 @default.
- W2079285846 cites W1984282081 @default.
- W2079285846 cites W1986665522 @default.
- W2079285846 cites W1986794853 @default.
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- W2079285846 cites W1992768136 @default.
- W2079285846 cites W1994892324 @default.
- W2079285846 cites W2001299036 @default.
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- W2079285846 cites W2005155920 @default.
- W2079285846 cites W2008562274 @default.
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- W2079285846 cites W2022121449 @default.
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- W2079285846 doi "https://doi.org/10.1111/j.1365-2990.2004.00578.x" @default.
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