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- W2079289660 abstract "Abstract In the present investigation, silica‐polyaniline based bienzyme cholesterol biosensor is fabricated through a simple one‐step electrochemical method. The one‐step fabrication process involves electrochemical polymerization of N[3‐(trimethoxysilyl)propyl]aniline to result poly(N[3‐(trimethoxysilyl)propyl]aniline) (PTMSPA) and simultaneous immobilization of two enzymes, horseradish peroxidase (HRP) and cholesterol oxidase (ChOx) into PTMSPA matrix. The modified electrode is designated as PTMSPA‐HRP/ChOx‐ME. PTMSPA facilitates direct electron transfer between the electrode surface and the active redox centers of HRP. This enables the operation of a biosensor at a low working potential of about −150 mV (vs. Ag/AgCl) for the detection of hydrogen peroxide. The PTMSPA‐HRP/ChOx‐ME demonstrates excellent analytical performance for the detection of cholesterol between 1 and 25 mM with high sensitivity and selectivity. PTMSPA possesses features suited for the fabrication of third‐generation biosensors." @default.
- W2079289660 created "2016-06-24" @default.
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- W2079289660 date "2010-08-31" @default.
- W2079289660 modified "2023-10-17" @default.
- W2079289660 title "Silica-Polyaniline Based Bienzyme Cholesterol Biosensor: Fabrication and Characterization" @default.
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- W2079289660 doi "https://doi.org/10.1002/elan.201000138" @default.
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