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- W2079293882 abstract "Unsensitized human lymphocytes damage allogeneic non-lymphoid cells in tissue culture, when incubated together with the latter in the presence of phytohemagglutinin (PHA). In previous publications evidence has been presented that cytotoxicity is exerted by immunologically competent lymphocytes. Close contact between lymphocytes and target cells is necessary for the reaction, and the stimulation of the lymphocytes induced by PHA increases the cytotoxic efficiency of the cells. The present report is concerned with the effect of metabolic inhibitors on the cytotoxicity of human lymphocytes on Chang cells (human liver). Cell damage was measured quantitatively as the release of isotope from Chang cells, labeled with 51Cr-chromate. Hydrocortisone (10–100 μg/ml) partially inhibited the cytotoxicity and reduced the PHA-induced lymphocyte stimulation. Inhibitors of protein synthesis (puromycin and parafluorophenylalanine) had no effect on the reaction. Actinomycin D (10 μg/ml) reduced RNA synthesis to a minimum but had no or only minor effects on cytotoxicity in 3–6 hr experiments. A late inhibition of cytotoxicity by actinomycin D was observed after 24 hr of incubation. This inhibition was probably caused by secondary toxic effects of the drug on the lymphocytes. Pretreatment of the lymphocytes at 43–45 °C efficiently reduced their cytotoxic activity. The cytotoxicity was inhibited by 10−3–10−2M iodoacetate which also impaired the anaerobic glycolysis of the lymphocytes. 10−2M 2,4-dinitrophenol abolished the cytotoxic reaction. Antimycin A inhibited the cytotoxicity in concentrations which impaired the respiration of the lymphocytes. The results are compatible with the assumption that the PHA-induced cytotoxicity of human lymphocytes depends on energy requiring processes but is independent of protein and RNA synthesis." @default.
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- W2079293882 date "1967-10-01" @default.
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- W2079293882 title "The in vitro cytotoxicity of human lymphocytes: The effect of metabolic inhibitors" @default.
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- W2079293882 doi "https://doi.org/10.1016/0014-4827(67)90359-x" @default.
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