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- W2079302544 abstract "Fresh rabbit serum inhibited basal glycogen deposition in mouse hemidiaphragms and reduced the glycogenic effect of 0.3 mU/ml insulin. This inhibitory effect was abolished by adrenalectomy. Dialysis of serum from fasting rabbit, dog, or man increased glycogen deposition in mouse hemidiaphragms in the presence or absence of insulin. This enhancing effect was due largely to enhancement of insulin action since most of it could be removed by addition of insulin antiserum. Insulin was added to fresh rabbit serum, dialyzed rabbit serum, and basal salts – gelatin solution and the immunoreactive insulin levels of the sera were measured before and after hemidiaphragms were incubated in them. Similar results were obtained for fresh and dialyzed sera, yet glycogen deposition was greater in hemidiaphragms incubated in dialyzed sera than in fresh sera or basal salts – gelatin solution. The enhanced effect was not due to differences in insulin removal. This was demonstrated also for dialyzed serum from depancreatized dogs. The presence in sera of factors affecting insulin action may introduce errors in biological assays. Standards must be made up in solutions resembling as closely as possible the unknown solution but lacking the substance under test." @default.
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- W2079302544 date "1972-06-01" @default.
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- W2079302544 title "Some Effects of Blood Sera on the Glycogenic Effects of Insulin on Muscle" @default.
- W2079302544 doi "https://doi.org/10.1139/y72-077" @default.
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