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• W2079306747 endingPage "3480" @default.
• W2079306747 startingPage "3475" @default.
• W2079306747 abstract "Disease conditions like malaria, sickle cell anemia, diabetes mellitus, cancer, etc., are known to significantly alter the deformability of certain types of cells (red blood cells, white blood cells, circulating tumor cells, etc.). To determine the cellular deformability, techniques like micropipette aspiration, atomic force microscopy, optical tweezers, quantitative phase imaging have been developed. Many of these techniques have an advantage of determining the single cell deformability with ultrahigh precision. However, the suitability of these techniques for the realization of a deformability based diagnostic tool is questionable as they are expensive and extremely slow to operate on a huge population of cells. In this paper, we propose a technique for high-throughput (800 cells/s) determination of cellular deformability on a single cell basis. This technique involves capturing the image(s) of cells in flow that have undergone deformation under the influence of shear gradient generated by the fluid flowing through the microfluidic channels. Deformability indices of these cells can be computed by performing morphological operations on these images. We demonstrate the applicability of this technique for examining the deformability index on healthy, diabetic, and sphered red blood cells. We believe that this technique has a strong role to play in the realization of a potential tool that uses deformability as one of the important criteria in disease diagnosis." @default.
• W2079306747 created "2016-06-24" @default.
• W2079306747 creator A5032579755 @default.
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• W2079306747 date "2015-08-15" @default.
• W2079306747 modified "2023-10-10" @default.
• W2079306747 title "Deformability Measurement of Single-Cells at High-Throughput With Imaging Flow Cytometry" @default.
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• W2079306747 doi "https://doi.org/10.1109/jlt.2015.2413834" @default.
• W2079306747 hasPublicationYear "2015" @default.
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