Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079313039> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2079313039 abstract "Monoclonal antibodies were raised against a purified membrane fraction from hog gastric mucosa containing H+/K(+)-ATPase. The properties of one of these monoclonal antibodies (5B6) were further evaluated. On immunoblot it recognized the 95 kDa peptide of the H+/K(+)-ATPase-rich membrane fraction. The K(+)-ATPase activity was inhibited by 65% under standard assay conditions (pH 7.0). At pH 6.0 and 8.0 this enzyme activity was inhibited by 40% and 100%, respectively. The maximal inhibition in inside-out vesicles was also 65% at pH 7.0. The inhibition was uncompetitive with respect to K+ and noncompetitive with respect to ATP. Mg2(+)-ATPase activity and K(+)-dependent p-nitrophenylphosphatase activity were not influenced. The monoclonal antibody lowered the steady-state phosphorylation level at pH 6.0, 7.0 and 8.0 by 30%, 40% and 60% respectively. The rate of the K(+)-stimulated dephosphorylation step was not inhibited. These findings demonstrate that 5-B6 recognizes the E1.K+ dephosphoenzyme at the cytosolic side." @default.
- W2079313039 created "2016-06-24" @default.
- W2079313039 creator A5039644025 @default.
- W2079313039 creator A5048880494 @default.
- W2079313039 creator A5059026194 @default.
- W2079313039 date "1990-03-01" @default.
- W2079313039 modified "2023-09-26" @default.
- W2079313039 title "A monoclonal antibody against pig gastric H+/K+-ATPase, which binds to the cytosolic E1·K+ form" @default.
- W2079313039 cites W1490607841 @default.
- W2079313039 cites W1506632686 @default.
- W2079313039 cites W1535311054 @default.
- W2079313039 cites W1550313514 @default.
- W2079313039 cites W1566584097 @default.
- W2079313039 cites W1578508637 @default.
- W2079313039 cites W1596185709 @default.
- W2079313039 cites W1602271438 @default.
- W2079313039 cites W1775749144 @default.
- W2079313039 cites W1975367964 @default.
- W2079313039 cites W1976717441 @default.
- W2079313039 cites W1986472994 @default.
- W2079313039 cites W1999792639 @default.
- W2079313039 cites W2000546733 @default.
- W2079313039 cites W2004099707 @default.
- W2079313039 cites W2006659213 @default.
- W2079313039 cites W2007300356 @default.
- W2079313039 cites W2008962068 @default.
- W2079313039 cites W2033111961 @default.
- W2079313039 cites W2046075488 @default.
- W2079313039 cites W2062801478 @default.
- W2079313039 cites W2064096290 @default.
- W2079313039 cites W2064210744 @default.
- W2079313039 cites W2064360694 @default.
- W2079313039 cites W2072327149 @default.
- W2079313039 cites W2077613046 @default.
- W2079313039 cites W2088782754 @default.
- W2079313039 cites W2100837269 @default.
- W2079313039 cites W2111817213 @default.
- W2079313039 cites W2179493408 @default.
- W2079313039 cites W2412060047 @default.
- W2079313039 doi "https://doi.org/10.1016/0005-2736(90)90009-d" @default.
- W2079313039 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2156563" @default.
- W2079313039 hasPublicationYear "1990" @default.
- W2079313039 type Work @default.
- W2079313039 sameAs 2079313039 @default.
- W2079313039 citedByCount "21" @default.
- W2079313039 crossrefType "journal-article" @default.
- W2079313039 hasAuthorship W2079313039A5039644025 @default.
- W2079313039 hasAuthorship W2079313039A5048880494 @default.
- W2079313039 hasAuthorship W2079313039A5059026194 @default.
- W2079313039 hasConcept C11960822 @default.
- W2079313039 hasConcept C130316041 @default.
- W2079313039 hasConcept C153911025 @default.
- W2079313039 hasConcept C159654299 @default.
- W2079313039 hasConcept C178666793 @default.
- W2079313039 hasConcept C181199279 @default.
- W2079313039 hasConcept C181912034 @default.
- W2079313039 hasConcept C185592680 @default.
- W2079313039 hasConcept C203014093 @default.
- W2079313039 hasConcept C23265538 @default.
- W2079313039 hasConcept C41625074 @default.
- W2079313039 hasConcept C542903549 @default.
- W2079313039 hasConcept C55493867 @default.
- W2079313039 hasConcept C86803240 @default.
- W2079313039 hasConcept C98539663 @default.
- W2079313039 hasConceptScore W2079313039C11960822 @default.
- W2079313039 hasConceptScore W2079313039C130316041 @default.
- W2079313039 hasConceptScore W2079313039C153911025 @default.
- W2079313039 hasConceptScore W2079313039C159654299 @default.
- W2079313039 hasConceptScore W2079313039C178666793 @default.
- W2079313039 hasConceptScore W2079313039C181199279 @default.
- W2079313039 hasConceptScore W2079313039C181912034 @default.
- W2079313039 hasConceptScore W2079313039C185592680 @default.
- W2079313039 hasConceptScore W2079313039C203014093 @default.
- W2079313039 hasConceptScore W2079313039C23265538 @default.
- W2079313039 hasConceptScore W2079313039C41625074 @default.
- W2079313039 hasConceptScore W2079313039C542903549 @default.
- W2079313039 hasConceptScore W2079313039C55493867 @default.
- W2079313039 hasConceptScore W2079313039C86803240 @default.
- W2079313039 hasConceptScore W2079313039C98539663 @default.
- W2079313039 hasLocation W20793130391 @default.
- W2079313039 hasLocation W20793130392 @default.
- W2079313039 hasOpenAccess W2079313039 @default.
- W2079313039 hasPrimaryLocation W20793130391 @default.
- W2079313039 hasRelatedWork W1496199639 @default.
- W2079313039 hasRelatedWork W1854217178 @default.
- W2079313039 hasRelatedWork W1987554033 @default.
- W2079313039 hasRelatedWork W1994765195 @default.
- W2079313039 hasRelatedWork W2034717845 @default.
- W2079313039 hasRelatedWork W2039011601 @default.
- W2079313039 hasRelatedWork W2072751246 @default.
- W2079313039 hasRelatedWork W2088999850 @default.
- W2079313039 hasRelatedWork W2091999937 @default.
- W2079313039 hasRelatedWork W2188844446 @default.
- W2079313039 isParatext "false" @default.
- W2079313039 isRetracted "false" @default.
- W2079313039 magId "2079313039" @default.
- W2079313039 workType "article" @default.