Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079324098> ?p ?o ?g. }
Showing items 1 to 83 of
83
with 100 items per page.
- W2079324098 endingPage "2473" @default.
- W2079324098 startingPage "2470" @default.
- W2079324098 abstract "Homologous desensitization of the ovarian LH-sensitive adenylyl cyclase in cell-free systems is known to be dependent on micromolar concentrations of GTP. In this study, we sought to determine whether the nucleotide requirement of GTP for desensitization is complete and specific. LH-dependent desensitization of the adenylyl cyclase of pig ovarian follicular membranes was examined without adding adenyl-5'-yl imidodiphosphate [AMP-P(NH)P] to prevent the degradation of nucleotide triphosphates. GTP at 0.1 mM or higher [in the absence of AMP-P(NH)P] was able to support the same amount of desensitization (30-35%) that occurs with 1 mM AMP-P(NH)P and micromolar concentrations of GTP. ATP, UTP, and CTP also supported maximal desensitization, but were 5-30 times less potent than GTP. The ED50 value for GTP (14 microM) was about 100 times higher than when the reaction was performed in the presence of 1 mM AMP-P(NH)P; the ED50 values for UTP (70 microM) and CTP and ATP (400 microM) were only 2-5 times higher than when AMP-P(NH)P was included. Incubation of [alpha-32P]GTP with pig follicular membranes demonstrated that 1 mM GTP was stable, whereas 10 microM or less was rapidly degraded unless 1 mM AMP-P(NH)P was included. The specificity of GTP for supporting desensitization was also demonstrated in experiments where guanyl-5'-yl imidodiphosphate, the nonhydrolyzable GTP analog, inhibited LH-induced desensitization which otherwise would have occurred in the presence of ATP, CTP, or UTP. These studies establish the complete and specific requirement of GTP for supporting desensitization of ovarian LH-responsive adenylyl cyclase." @default.
- W2079324098 created "2016-06-24" @default.
- W2079324098 creator A5063798407 @default.
- W2079324098 creator A5065856781 @default.
- W2079324098 date "1989-11-01" @default.
- W2079324098 modified "2023-09-26" @default.
- W2079324098 title "Guanosine Triphosphate Fulfills a Complete and Specific Nucleotide Requirement for Luteinizing Hormone-Induced Desensitization of Pig Ovarian Adenylyl Cyclase*" @default.
- W2079324098 cites W1513523543 @default.
- W2079324098 cites W1589012184 @default.
- W2079324098 cites W1987864027 @default.
- W2079324098 cites W2014863394 @default.
- W2079324098 cites W2020349910 @default.
- W2079324098 cites W2067968725 @default.
- W2079324098 cites W2110477606 @default.
- W2079324098 cites W2170585738 @default.
- W2079324098 cites W2412082992 @default.
- W2079324098 cites W2418352942 @default.
- W2079324098 doi "https://doi.org/10.1210/endo-125-5-2470" @default.
- W2079324098 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2791997" @default.
- W2079324098 hasPublicationYear "1989" @default.
- W2079324098 type Work @default.
- W2079324098 sameAs 2079324098 @default.
- W2079324098 citedByCount "25" @default.
- W2079324098 countsByYear W20793240982012 @default.
- W2079324098 crossrefType "journal-article" @default.
- W2079324098 hasAuthorship W2079324098A5063798407 @default.
- W2079324098 hasAuthorship W2079324098A5065856781 @default.
- W2079324098 hasConcept C104317684 @default.
- W2079324098 hasConcept C118716 @default.
- W2079324098 hasConcept C126322002 @default.
- W2079324098 hasConcept C134018914 @default.
- W2079324098 hasConcept C170493617 @default.
- W2079324098 hasConcept C181199279 @default.
- W2079324098 hasConcept C185592680 @default.
- W2079324098 hasConcept C24998067 @default.
- W2079324098 hasConcept C2776638253 @default.
- W2079324098 hasConcept C2777995097 @default.
- W2079324098 hasConcept C2779178603 @default.
- W2079324098 hasConcept C2779779143 @default.
- W2079324098 hasConcept C512185932 @default.
- W2079324098 hasConcept C55493867 @default.
- W2079324098 hasConcept C71924100 @default.
- W2079324098 hasConcept C86803240 @default.
- W2079324098 hasConcept C92759361 @default.
- W2079324098 hasConceptScore W2079324098C104317684 @default.
- W2079324098 hasConceptScore W2079324098C118716 @default.
- W2079324098 hasConceptScore W2079324098C126322002 @default.
- W2079324098 hasConceptScore W2079324098C134018914 @default.
- W2079324098 hasConceptScore W2079324098C170493617 @default.
- W2079324098 hasConceptScore W2079324098C181199279 @default.
- W2079324098 hasConceptScore W2079324098C185592680 @default.
- W2079324098 hasConceptScore W2079324098C24998067 @default.
- W2079324098 hasConceptScore W2079324098C2776638253 @default.
- W2079324098 hasConceptScore W2079324098C2777995097 @default.
- W2079324098 hasConceptScore W2079324098C2779178603 @default.
- W2079324098 hasConceptScore W2079324098C2779779143 @default.
- W2079324098 hasConceptScore W2079324098C512185932 @default.
- W2079324098 hasConceptScore W2079324098C55493867 @default.
- W2079324098 hasConceptScore W2079324098C71924100 @default.
- W2079324098 hasConceptScore W2079324098C86803240 @default.
- W2079324098 hasConceptScore W2079324098C92759361 @default.
- W2079324098 hasIssue "5" @default.
- W2079324098 hasLocation W20793240981 @default.
- W2079324098 hasLocation W20793240982 @default.
- W2079324098 hasOpenAccess W2079324098 @default.
- W2079324098 hasPrimaryLocation W20793240981 @default.
- W2079324098 hasRelatedWork W1967443949 @default.
- W2079324098 hasRelatedWork W1967664991 @default.
- W2079324098 hasRelatedWork W1976662905 @default.
- W2079324098 hasRelatedWork W2007514289 @default.
- W2079324098 hasRelatedWork W2079324098 @default.
- W2079324098 hasRelatedWork W2129497664 @default.
- W2079324098 hasRelatedWork W2130310738 @default.
- W2079324098 hasRelatedWork W2194300488 @default.
- W2079324098 hasRelatedWork W2414300509 @default.
- W2079324098 hasRelatedWork W979752654 @default.
- W2079324098 hasVolume "125" @default.
- W2079324098 isParatext "false" @default.
- W2079324098 isRetracted "false" @default.
- W2079324098 magId "2079324098" @default.
- W2079324098 workType "article" @default.