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• W2079343462 abstract "Abstract Binding of the chromogenic ligand p -nitrophenyl α- d -mannopyranoside to concanavalin A was studied in a stopped-flow spectrometer. Formation of the protein-ligand complex could be represented as a simple one-step process. No kinetic evidence could be obtained for a ligand-induced change in the conformation of concanavalin A, although the existence of such a conformational change was not excluded. The entire change in absorbance produced on ligand binding occurred in the monophasic process monitored in the stopped-flow spectrometer. The value of the apparent second-order rate constant ( k a ) for complex formation ( k a = 54,000 s −1 m − at 25 °C, pH 5.0, Γ/2 0.5) was independent of the protein concentration when the protein was in the range of 233–831 μ m in combining sites and in excess of the ligand. The apparent first-order rate constant ( k −a ) for dissociation of the complex was obtained from the rate constant for the decomposition of the complex upon the addition of excess methyl α- d -mannopyranoside ( k −a = 6.2 s −1 at 25 °C, pH 5.0, Γ/2 0.5). The ratio k a − a (0.9 × 10 4 m −1 ) was in reasonable agreement with value of 1.1 ± 0.1 × 10 4 m −1 determined for the equilibrium constant for complex formation by ultraviolet difference spectrometry. Plots of ln( k a T ) and ln( k a T ) vs 1 T were linear ( T is temperature) and were used to evaluate activation parameters. The enthalpies of activation for formation and dissociation of the complex are 9.5 ± 0.3 and 16.8 ± 0.2 kcal/mol, respectively. The unitary entropies of activation for formation and dissociation of the complex are 2.8 ± 1.1 and 1.3 ± 0.7 entropy units, respectively. These entropy changes are much less than those usually associated with substantial changes in the conformation of proteins." @default.
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• W2079343462 date "1976-02-01" @default.
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• W2079343462 title "Kinetic parameters for the binding of p-nitrophenyl α-d-mannopyranoside to concanavalin A" @default.
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• W2079343462 doi "https://doi.org/10.1016/0003-9861(76)90125-9" @default.
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