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- W2079354926 abstract "The preferred dye binding sites and the microenvironment of known nucleotide sequences within mitochondrial and plasmid pBR322 DNA was probed in a gross fashion with restriction endonucleases. The intercalating dyes, ethidium bromide and propidium iodide, do not inhibit a given restriction endonuclease equally at all of the restriction sites within a DNA molecule. The selective inhibition may be explained, in part, by the potential B to Z conformation transition of DNA flanking the restriction site and by preferred dye binding sites. Propidium iodide was found to be a more potent inhibitor than ethidium bromide and the inhibition is independent of the type of cut made by the enzyme." @default.
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- W2079354926 date "1983-09-01" @default.
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- W2079354926 title "Selective inhibition of restriction endonuclease cleavage by DNA intercalators" @default.
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- W2079354926 doi "https://doi.org/10.1016/s0006-291x(83)80170-3" @default.
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