FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079357192> ?p ?o ?g. }

• W2079357192 endingPage "22" @default.
• W2079357192 startingPage "14" @default.
• W2079357192 abstract "Myeloid-derived suppressor cells (MDSC) present in the human peripheral blood, represent a heterogeneous population of cells with monocytic and granulocytic features. To provide guidelines for reliable assessments of the frequency and function of MDSC, we compared fresh vs. cryopreserved peripheral blood mononuclear cell (PBMC) samples obtained from normal controls and patients with cancer. PBMC were obtained from 4 healthy donors and 21 patients with cancer. They were stained with labeled antibodies, and the frequency of DR−/LIN−/CD11b+, DR−/LIN−/CD15+, DR−/LIN−/CD33+ and DR−/low/CD14+ cells was determined by flow cytometry before and after cryopreservation. CFSE-based suppressor assays were used to test inhibitory functions of MDSC. Arginase I expression and reactive oxygen species (ROS) upregulation in MDSC subsets were evaluated by flow cytometry. The DR−/low/CD14+ and DR−/LIN−/CD11b+ subsets of MDSC were found to be more resistant to the cryopreservation/thawing procedure compared to the DR−/LIN−/CD15+ and DR−/LIN−/CD33+ subsets. The frequency of the latter two MDSC subsets was significantly reduced after cryopreservation. All but DR−/LIN−/CD15+ cells inhibited proliferation of autologous CSFE-labeled CD4+ cells but lost suppressor activity after cryopreservation. Only DR−/LIN−/CD15+ cells were positive for Arginase I, but lost its expression after cryopreservation. Only fresh DR−/LIN−/CD11b+ and DR−/LIN−/CD15+ cells produced ROS after in vitro stimulation. Studies of human MDSC should be performed in fresh blood samples. If samples have to be cryopreserved, monitoring of CD11b+ and CD14+ MDSC subsets provides the most reliable results. Arginase I expression or stimulated ROS production assessed by flow cytometry are useful markers for MDSC subsets only in fresh samples." @default.
• W2079357192 created "2016-06-24" @default.
• W2079357192 creator A5008750058 @default.
• W2079357192 creator A5008924495 @default.
• W2079357192 creator A5014087122 @default.
• W2079357192 creator A5018141014 @default.
• W2079357192 creator A5050172772 @default.
• W2079357192 creator A5052666093 @default.
• W2079357192 date "2012-07-01" @default.
• W2079357192 modified "2023-10-17" @default.
• W2079357192 title "Myeloid-derived suppressor cell measurements in fresh and cryopreserved blood samples" @default.
• W2079357192 cites W1580678794 @default.
• W2079357192 cites W1585754595 @default.
• W2079357192 cites W1598162705 @default.
• W2079357192 cites W1925817305 @default.
• W2079357192 cites W1960585934 @default.
• W2079357192 cites W1975191869 @default.
• W2079357192 cites W1976310228 @default.
• W2079357192 cites W2011627030 @default.
• W2079357192 cites W2023389612 @default.
• W2079357192 cites W2030338021 @default.
• W2079357192 cites W2031200699 @default.
• W2079357192 cites W2038918212 @default.
• W2079357192 cites W2059688175 @default.
• W2079357192 cites W2060902798 @default.
• W2079357192 cites W2068994108 @default.
• W2079357192 cites W2082413924 @default.
• W2079357192 cites W2092639400 @default.
• W2079357192 cites W2113266244 @default.
• W2079357192 cites W2117933504 @default.
• W2079357192 cites W2119077297 @default.
• W2079357192 cites W2128957395 @default.
• W2079357192 cites W2134401635 @default.
• W2079357192 cites W2134459266 @default.
• W2079357192 cites W2146437446 @default.
• W2079357192 cites W2158853057 @default.
• W2079357192 cites W2165330900 @default.
• W2079357192 doi "https://doi.org/10.1016/j.jim.2012.04.004" @default.
• W2079357192 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3385927" @default.
• W2079357192 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22522114" @default.
• W2079357192 hasPublicationYear "2012" @default.
• W2079357192 type Work @default.
• W2079357192 sameAs 2079357192 @default.
• W2079357192 citedByCount "162" @default.
• W2079357192 countsByYear W20793571922012 @default.
• W2079357192 countsByYear W20793571922013 @default.
• W2079357192 countsByYear W20793571922014 @default.
• W2079357192 countsByYear W20793571922015 @default.
• W2079357192 countsByYear W20793571922016 @default.
• W2079357192 countsByYear W20793571922017 @default.
• W2079357192 countsByYear W20793571922018 @default.
• W2079357192 countsByYear W20793571922019 @default.
• W2079357192 countsByYear W20793571922020 @default.
• W2079357192 countsByYear W20793571922021 @default.
• W2079357192 countsByYear W20793571922022 @default.
• W2079357192 countsByYear W20793571922023 @default.
• W2079357192 crossrefType "journal-article" @default.
• W2079357192 hasAuthorship W2079357192A5008750058 @default.
• W2079357192 hasAuthorship W2079357192A5008924495 @default.
• W2079357192 hasAuthorship W2079357192A5014087122 @default.
• W2079357192 hasAuthorship W2079357192A5018141014 @default.
• W2079357192 hasAuthorship W2079357192A5050172772 @default.
• W2079357192 hasAuthorship W2079357192A5052666093 @default.
• W2079357192 hasBestOaLocation W20793571922 @default.
• W2079357192 hasConcept C10205521 @default.
• W2079357192 hasConcept C121608353 @default.
• W2079357192 hasConcept C126322002 @default.
• W2079357192 hasConcept C137061746 @default.
• W2079357192 hasConcept C153911025 @default.
• W2079357192 hasConcept C16685009 @default.
• W2079357192 hasConcept C171779818 @default.
• W2079357192 hasConcept C176290653 @default.
• W2079357192 hasConcept C179185449 @default.
• W2079357192 hasConcept C179933525 @default.
• W2079357192 hasConcept C185592680 @default.
• W2079357192 hasConcept C196843134 @default.
• W2079357192 hasConcept C202751555 @default.
• W2079357192 hasConcept C203014093 @default.
• W2079357192 hasConcept C2776090121 @default.
• W2079357192 hasConcept C2776804306 @default.
• W2079357192 hasConcept C2778513237 @default.
• W2079357192 hasConcept C28328180 @default.
• W2079357192 hasConcept C2908647359 @default.
• W2079357192 hasConcept C553184892 @default.
• W2079357192 hasConcept C55493867 @default.
• W2079357192 hasConcept C55721878 @default.
• W2079357192 hasConcept C71924100 @default.
• W2079357192 hasConcept C86803240 @default.
• W2079357192 hasConcept C8891405 @default.
• W2079357192 hasConcept C95444343 @default.
• W2079357192 hasConcept C99454951 @default.
• W2079357192 hasConceptScore W2079357192C10205521 @default.
• W2079357192 hasConceptScore W2079357192C121608353 @default.
• W2079357192 hasConceptScore W2079357192C126322002 @default.
• W2079357192 hasConceptScore W2079357192C137061746 @default.
• W2079357192 hasConceptScore W2079357192C153911025 @default.
• W2079357192 hasConceptScore W2079357192C16685009 @default.
• W2079357192 hasConceptScore W2079357192C171779818 @default.

• next