Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079365545> ?p ?o ?g. }
- W2079365545 endingPage "814" @default.
- W2079365545 startingPage "801" @default.
- W2079365545 abstract "Parkinson's disease (PD) is a common neurodegenerative disease characterized by the selective loss of dopaminergic (DA) neurons in the midbrain. Various types of stem cells that have potential to differentiate into DA neurons are being investigated as cellular therapies for PD. Stem cells also secrete growth factors and therefore also may have therapeutic effects in promoting the health of diseased DA neurons in the PD brain. To address this possibility in an experimental model of PD, bone marrow-derived neuroprogenitor-like cells were generated from bone marrow procured from healthy human adult volunteers and their potential to elicit recovery of damaged DA axons was studied in a partial lesion rat model of PD. Following collection of bone marrow, mesenchymal stem cells (MSC) were isolated and then genetically modified to create SB623 cells by transient transfection with the intracellular domain of the Notch1 gene (NICD), a modification that upregulates expression of certain neuroprogenitor markers. Ten deposits of 0.5 μl of SB623 cell suspension adjusted from 6,000 to 21,000 cells/μl in PBS or PBS alone were stereotaxically placed in the striatum 1 week after the nigrostriatal projection had been partially lesioned in adult F344 rats by injection of 6-hydroxydopamine (6-OHDA) into the striatum. At 3 weeks, a small number of grafted SB623 cells survived in the lesioned striatum as visualized by expression of the human specific nuclear matrix protein (hNuMA). In rats that received SB623 cells, but not in control rats, dense tyrosine hydroxylase immunoreactive (TH-ir) fibers were observed around the grafts. These fibers appeared to be rejuvenated host DA axons because no TH-ir in soma of surviving SB623 cells or coexpression of TH and hNuMA-ir were observed. In addition, dense serotonin immunoreactive (5-HT-ir) fibers were observed around grafted SB623 cells and these fibers also appeared to be of the host origin. Also, in some SB623 grafted rats that were sacrificed within 2 h of dl-amphetamine injection, hot spots of c-Fos-positive nuclei that coincided with rejuvenated dense TH fibers around the grafted SB623 cells were observed, suggesting increased availability of DA in these locations. Our observations suggest that NICD-transfected MSC hold potential as a readily available autologous or allogenic cellular therapy for ameliorating the degeneration of DA and 5-HT neurons in PD patients." @default.
- W2079365545 created "2016-06-24" @default.
- W2079365545 creator A5005832685 @default.
- W2079365545 creator A5012991123 @default.
- W2079365545 creator A5027781323 @default.
- W2079365545 creator A5046883964 @default.
- W2079365545 creator A5077906972 @default.
- W2079365545 creator A5081070880 @default.
- W2079365545 creator A5084283465 @default.
- W2079365545 creator A5089736596 @default.
- W2079365545 date "2009-07-01" @default.
- W2079365545 modified "2023-09-24" @default.
- W2079365545 title "Reversal of Dopaminergic Degeneration in a Parkinsonian Rat following Micrografting of Human Bone Marrow-Derived Neural Progenitors" @default.
- W2079365545 cites W1519240899 @default.
- W2079365545 cites W1643991689 @default.
- W2079365545 cites W1804407417 @default.
- W2079365545 cites W1924525408 @default.
- W2079365545 cites W1970007383 @default.
- W2079365545 cites W1971017960 @default.
- W2079365545 cites W1973028673 @default.
- W2079365545 cites W1981287437 @default.
- W2079365545 cites W1985069671 @default.
- W2079365545 cites W1985508547 @default.
- W2079365545 cites W1986396861 @default.
- W2079365545 cites W1989720524 @default.
- W2079365545 cites W1991062038 @default.
- W2079365545 cites W1992618202 @default.
- W2079365545 cites W2001361641 @default.
- W2079365545 cites W2001477976 @default.
- W2079365545 cites W2005657014 @default.
- W2079365545 cites W2005848913 @default.
- W2079365545 cites W2007890661 @default.
- W2079365545 cites W2010838675 @default.
- W2079365545 cites W2011823445 @default.
- W2079365545 cites W2012658246 @default.
- W2079365545 cites W2015500392 @default.
- W2079365545 cites W2026246891 @default.
- W2079365545 cites W2027197900 @default.
- W2079365545 cites W2028311590 @default.
- W2079365545 cites W2032114206 @default.
- W2079365545 cites W2032281007 @default.
- W2079365545 cites W2037962004 @default.
- W2079365545 cites W2038197168 @default.
- W2079365545 cites W2038986435 @default.
- W2079365545 cites W2039836896 @default.
- W2079365545 cites W2047455245 @default.
- W2079365545 cites W2049393626 @default.
- W2079365545 cites W2051021067 @default.
- W2079365545 cites W2052956756 @default.
- W2079365545 cites W2054507508 @default.
- W2079365545 cites W2057159662 @default.
- W2079365545 cites W2059066863 @default.
- W2079365545 cites W2062118159 @default.
- W2079365545 cites W2064247269 @default.
- W2079365545 cites W2065000444 @default.
- W2079365545 cites W2066349985 @default.
- W2079365545 cites W2069170243 @default.
- W2079365545 cites W2071360006 @default.
- W2079365545 cites W2074997282 @default.
- W2079365545 cites W2079014632 @default.
- W2079365545 cites W2079365338 @default.
- W2079365545 cites W2079883031 @default.
- W2079365545 cites W2079935484 @default.
- W2079365545 cites W2087081786 @default.
- W2079365545 cites W2087665223 @default.
- W2079365545 cites W2088218095 @default.
- W2079365545 cites W2089839531 @default.
- W2079365545 cites W2092805385 @default.
- W2079365545 cites W2093377304 @default.
- W2079365545 cites W2093519473 @default.
- W2079365545 cites W2094635970 @default.
- W2079365545 cites W2100975089 @default.
- W2079365545 cites W2104793567 @default.
- W2079365545 cites W2108724455 @default.
- W2079365545 cites W2116500923 @default.
- W2079365545 cites W2138894342 @default.
- W2079365545 cites W2142594288 @default.
- W2079365545 cites W2145050029 @default.
- W2079365545 cites W2147015050 @default.
- W2079365545 cites W2148841089 @default.
- W2079365545 cites W2150857721 @default.
- W2079365545 cites W2150892075 @default.
- W2079365545 cites W2158048826 @default.
- W2079365545 cites W2159999899 @default.
- W2079365545 cites W2326545304 @default.
- W2079365545 cites W2423513091 @default.
- W2079365545 cites W4244311449 @default.
- W2079365545 doi "https://doi.org/10.3727/096368909x470801" @default.
- W2079365545 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19796495" @default.
- W2079365545 hasPublicationYear "2009" @default.
- W2079365545 type Work @default.
- W2079365545 sameAs 2079365545 @default.
- W2079365545 citedByCount "57" @default.
- W2079365545 countsByYear W20793655452012 @default.
- W2079365545 countsByYear W20793655452013 @default.
- W2079365545 countsByYear W20793655452014 @default.
- W2079365545 countsByYear W20793655452015 @default.
- W2079365545 countsByYear W20793655452016 @default.