Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079372923> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W2079372923 endingPage "61" @default.
- W2079372923 startingPage "49" @default.
- W2079372923 abstract "1.|Carotene 15,15′-dioxygenase (EC 1.13.11.21) has been isolated from the intestine of guinea pig and rabbit and purified 38- and 30-fold, respectively, but subjecting the intestinal homogenate to protamine sulfate treatment, (NH4)2SO4 fractionation and acetone precipitation. 2.|The guinea pig enzyme showed a pH optimum at 8.5, an optimum substrate concentration of 200 nmoles of β,β-carotene per 25 ml of reaction mixture, an apparent Km with β,β-carotene as substrate of 9.5 · 10−6 M and a V 3.3 nmoles of retinal formation/mg protein per h. The reaction was linear upto 3 h and the reaction rate increased linearly with increase in enzyme protein concentration. The enzyme was activated by GSH and Fe2+ and inhibited by sodium dodecylsulfate, sulfhydryl binding and iron chelating agents. 3.|The reaction catalysed by guinea pig enzyme was strictly stoichiometric. 4.|Rabbit enzyme showed a close similarity with guinea pig enzyme with respect to time course, optimum substrate concentration, activation by Fe2+ and GSH, inhibition by sodium dodecylsulfate, iron chelating and sulfhydryl binding agents. However, it showed a slightly lower pH optimum (pH 7.8). 5.|The enzyme from guinea pig and rabbit showed remarkable similarity with respect to cleavage of carotenoids. The enzyme from both the species was more specific for β,β-carotene but could also cleave a number of other carotenoids at the 15,15′-double bond. 6.|10′-Apo-β-carotenal and 10′-apo-β-carotenol were readily cleaved compared with other apo-β-carotenals and apo-β-carotenols tested. 7.|It has been conclusively shown for the first time that mono-ring substituted carotenoids are also cleaved at the 15,15′-double bond." @default.
- W2079372923 created "2016-06-24" @default.
- W2079372923 creator A5000414353 @default.
- W2079372923 creator A5049698023 @default.
- W2079372923 date "1974-11-01" @default.
- W2079372923 modified "2023-09-27" @default.
- W2079372923 title "Enzymatic cleavage of carotenoids" @default.
- W2079372923 cites W1562286640 @default.
- W2079372923 cites W1775749144 @default.
- W2079372923 cites W181832661 @default.
- W2079372923 cites W1870140516 @default.
- W2079372923 cites W1995256898 @default.
- W2079372923 cites W2061997861 @default.
- W2079372923 cites W2062832978 @default.
- W2079372923 cites W2063639389 @default.
- W2079372923 cites W2281120189 @default.
- W2079372923 cites W2296834060 @default.
- W2079372923 cites W2334539973 @default.
- W2079372923 cites W2336397498 @default.
- W2079372923 cites W2398539175 @default.
- W2079372923 cites W2404129495 @default.
- W2079372923 cites W57770207 @default.
- W2079372923 cites W77707196 @default.
- W2079372923 doi "https://doi.org/10.1016/0005-2744(74)90030-8" @default.
- W2079372923 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4429707" @default.
- W2079372923 hasPublicationYear "1974" @default.
- W2079372923 type Work @default.
- W2079372923 sameAs 2079372923 @default.
- W2079372923 citedByCount "55" @default.
- W2079372923 countsByYear W20793729232012 @default.
- W2079372923 countsByYear W20793729232013 @default.
- W2079372923 countsByYear W20793729232016 @default.
- W2079372923 countsByYear W20793729232017 @default.
- W2079372923 countsByYear W20793729232018 @default.
- W2079372923 countsByYear W20793729232020 @default.
- W2079372923 countsByYear W20793729232022 @default.
- W2079372923 crossrefType "journal-article" @default.
- W2079372923 hasAuthorship W2079372923A5000414353 @default.
- W2079372923 hasAuthorship W2079372923A5049698023 @default.
- W2079372923 hasConcept C181199279 @default.
- W2079372923 hasConcept C185592680 @default.
- W2079372923 hasConcept C18903297 @default.
- W2079372923 hasConcept C2777289219 @default.
- W2079372923 hasConcept C2781213060 @default.
- W2079372923 hasConcept C43617362 @default.
- W2079372923 hasConcept C55493867 @default.
- W2079372923 hasConcept C86803240 @default.
- W2079372923 hasConceptScore W2079372923C181199279 @default.
- W2079372923 hasConceptScore W2079372923C185592680 @default.
- W2079372923 hasConceptScore W2079372923C18903297 @default.
- W2079372923 hasConceptScore W2079372923C2777289219 @default.
- W2079372923 hasConceptScore W2079372923C2781213060 @default.
- W2079372923 hasConceptScore W2079372923C43617362 @default.
- W2079372923 hasConceptScore W2079372923C55493867 @default.
- W2079372923 hasConceptScore W2079372923C86803240 @default.
- W2079372923 hasIssue "1" @default.
- W2079372923 hasLocation W20793729231 @default.
- W2079372923 hasLocation W20793729232 @default.
- W2079372923 hasOpenAccess W2079372923 @default.
- W2079372923 hasPrimaryLocation W20793729231 @default.
- W2079372923 hasRelatedWork W1976637665 @default.
- W2079372923 hasRelatedWork W2056980537 @default.
- W2079372923 hasRelatedWork W2119490294 @default.
- W2079372923 hasRelatedWork W2155087996 @default.
- W2079372923 hasRelatedWork W2379318974 @default.
- W2079372923 hasRelatedWork W2404382196 @default.
- W2079372923 hasRelatedWork W2949708076 @default.
- W2079372923 hasRelatedWork W2951025516 @default.
- W2079372923 hasRelatedWork W2952203867 @default.
- W2079372923 hasRelatedWork W2952529774 @default.
- W2079372923 hasVolume "370" @default.
- W2079372923 isParatext "false" @default.
- W2079372923 isRetracted "false" @default.
- W2079372923 magId "2079372923" @default.
- W2079372923 workType "article" @default.