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- W2079390987 abstract "ABSTRACT We have cloned a Candida albicans gene ( CaMIG1 ) that encodes a protein homologous to the DNA-binding protein Mig1 from Saccharomyces cerevisiae ( Sc Mig1). The C. albicans Mig1 protein ( Ca Mig1) differs from Sc Mig1, in that, among other things, it lacks a putative phosphorylation site for Snf1 and presents several long stretches rich in glutamine or in asparagine, serine, and threonine and has the effector domain located at some distance (50 amino acids) from the carboxy terminus. Expression of CaMIG1 was low and was similar in glucose-, sucrose-, or ethanol-containing media. Disruption of the two CaMIG1 genomic copies had no effect in filamentation or infectivity. Levels of a glucose-repressible α-glucosidase, implicated in both sucrose and maltose utilization, were similar in wild-type or mig1/mig1 cells. Disruption of CaMIG1 had also no effect on the expression of the glucose-repressed gene CaGAL1. CaMIG1 was functional in S. cerevisiae , as judged by its ability to suppress the phenotypes produced by mig1 or tps1 mutations. In addition, Ca Mig1 formed specific complexes with the URS1 region of the S. cerevisiae FBP1 gene. The existence of a possible functional analogue of CaMIG1 in C. albicans was suggested by the results of band shift experiments." @default.
- W2079390987 created "2016-06-24" @default.
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- W2079390987 date "2000-01-15" @default.
- W2079390987 modified "2023-09-27" @default.
- W2079390987 title "Isolation of the <i>MIG1</i> Gene from <i>Candida albicans</i> and Effects of Its Disruption on Catabolite Repression" @default.
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- W2079390987 doi "https://doi.org/10.1128/jb.182.2.320-326.2000" @default.
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