FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079393806> ?p ?o ?g. }

• W2079393806 endingPage "288" @default.
• W2079393806 startingPage "271" @default.
• W2079393806 abstract "Monoclonal antibody (MAb) technology was used to examine aggrecan metabolites and the role of aggrecanases and matrix metalloproteinases (MMPs) in proteolysis of the interglobular domain (IGD) and C-terminus of aggrecan. An in vitro model of progressive cartilage degradation characterized by early proteoglycan loss and late stage collagen catabolism was evaluated in conjunction with a broad-spectrum inhibitor of MMPs. We have for the first time demonstrated that IGD cleavage by MMPs occurs during this late stage cartilage degeneration, both as a primary event in association with glycosaminoglycan (GAG) release from the tissue and secondarily in trimming of aggrecanase-generated G1 metabolites. Additionally, we have shown that MMPs were responsible for C-terminal catabolism of aggrecan and generation of chondroitin sulfate (CS) deficient aggrecan monomers and that this aggrecan truncation occurred prior to detectable IGD cleavage by MMPs. The onset of this later stage MMP activity was also evident by the generation of MMP-specific link protein catabolites in this model culture system. Recombinant MMP-1, -3 and -13 were all capable of C-terminally truncating aggrecan with at least two cleavage sites N-terminal to the CS attachment domains of aggrecan. Through analysis of aggrecan metabolites in pathological synovial fluids from human, canine and equine sources, we have demonstrated the presence of aggrecan catabolites that appear to have resulted from similar C-terminal processing of aggrecan as that induced in our in vitro culture systems. Finally, by developing a new MAb recognizing a linear epitope in the IGD of aggrecan, we have identified two novel aggrecan metabolites generated by an as yet unidentified proteolytic event. Collectively, these results suggest that C-terminal processing of aggrecan by MMPs may contribute to the depletion of cartilage GAG that leads to loss of tissue function in aging and disease. Furthermore, analysis of aggrecan metabolites resulting from both C-terminal and IGD cleavage by MMPs may prove useful in monitoring different stages in the progression of cartilage degeneration." @default.
• W2079393806 created "2016-06-24" @default.
• W2079393806 creator A5012058280 @default.
• W2079393806 creator A5012474280 @default.
• W2079393806 creator A5023553044 @default.
• W2079393806 creator A5027703531 @default.
• W2079393806 creator A5029535559 @default.
• W2079393806 creator A5037596314 @default.
• W2079393806 creator A5047486371 @default.
• W2079393806 creator A5070019687 @default.
• W2079393806 creator A5074407227 @default.
• W2079393806 creator A5077587176 @default.
• W2079393806 creator A5079387565 @default.
• W2079393806 date "2002-04-01" @default.
• W2079393806 modified "2023-10-18" @default.
• W2079393806 title "Matrix metalloproteinases are involved in C-terminal and interglobular domain processing of cartilage aggrecan in late stage cartilage degradation" @default.
• W2079393806 cites W109076851 @default.
• W2079393806 cites W1272499475 @default.
• W2079393806 cites W1486701492 @default.
• W2079393806 cites W1490074841 @default.
• W2079393806 cites W1494656326 @default.
• W2079393806 cites W1533521663 @default.
• W2079393806 cites W1581640555 @default.
• W2079393806 cites W1588094592 @default.
• W2079393806 cites W1615883506 @default.
• W2079393806 cites W1628236543 @default.
• W2079393806 cites W1712774648 @default.
• W2079393806 cites W178753903 @default.
• W2079393806 cites W1808369276 @default.
• W2079393806 cites W1818505718 @default.
• W2079393806 cites W1874814209 @default.
• W2079393806 cites W1878525374 @default.
• W2079393806 cites W1973897421 @default.
• W2079393806 cites W1987606644 @default.
• W2079393806 cites W1992130282 @default.
• W2079393806 cites W1992837858 @default.
• W2079393806 cites W1999307856 @default.
• W2079393806 cites W2003867975 @default.
• W2079393806 cites W2007417454 @default.
• W2079393806 cites W2007877800 @default.
• W2079393806 cites W2009581109 @default.
• W2079393806 cites W2010837855 @default.
• W2079393806 cites W2017045877 @default.
• W2079393806 cites W2023560282 @default.
• W2079393806 cites W2028313997 @default.
• W2079393806 cites W2032255522 @default.
• W2079393806 cites W2032541805 @default.
• W2079393806 cites W2034919108 @default.
• W2079393806 cites W2037270622 @default.
• W2079393806 cites W2040990919 @default.
• W2079393806 cites W2045503348 @default.
• W2079393806 cites W2051102442 @default.
• W2079393806 cites W2055034098 @default.
• W2079393806 cites W2056977500 @default.
• W2079393806 cites W2063571042 @default.
• W2079393806 cites W2069448431 @default.
• W2079393806 cites W2070952973 @default.
• W2079393806 cites W2074016505 @default.
• W2079393806 cites W2081694240 @default.
• W2079393806 cites W2081969734 @default.
• W2079393806 cites W2082794471 @default.
• W2079393806 cites W2084347324 @default.
• W2079393806 cites W2086013842 @default.
• W2079393806 cites W2087193732 @default.
• W2079393806 cites W2088930264 @default.
• W2079393806 cites W2089378618 @default.
• W2079393806 cites W2089687988 @default.
• W2079393806 cites W2090695665 @default.
• W2079393806 cites W2103691455 @default.
• W2079393806 cites W2106862840 @default.
• W2079393806 cites W2114769400 @default.
• W2079393806 cites W2116013040 @default.
• W2079393806 cites W2133558852 @default.
• W2079393806 cites W2144347714 @default.
• W2079393806 cites W2150828068 @default.
• W2079393806 cites W2167869180 @default.
• W2079393806 cites W2210166980 @default.
• W2079393806 cites W2236998588 @default.
• W2079393806 cites W2256063435 @default.
• W2079393806 cites W2339417578 @default.
• W2079393806 cites W4232925012 @default.
• W2079393806 cites W4237440792 @default.
• W2079393806 cites W4238590752 @default.
• W2079393806 doi "https://doi.org/10.1016/s0945-053x(02)00004-5" @default.
• W2079393806 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12009333" @default.
• W2079393806 hasPublicationYear "2002" @default.
• W2079393806 type Work @default.
• W2079393806 sameAs 2079393806 @default.
• W2079393806 citedByCount "117" @default.
• W2079393806 countsByYear W20793938062012 @default.
• W2079393806 countsByYear W20793938062013 @default.
• W2079393806 countsByYear W20793938062014 @default.
• W2079393806 countsByYear W20793938062015 @default.
• W2079393806 countsByYear W20793938062016 @default.
• W2079393806 countsByYear W20793938062017 @default.
• W2079393806 countsByYear W20793938062018 @default.
• W2079393806 countsByYear W20793938062019 @default.
• W2079393806 countsByYear W20793938062020 @default.

• next