Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079394992> ?p ?o ?g. }
- W2079394992 endingPage "13098" @default.
- W2079394992 startingPage "13093" @default.
- W2079394992 abstract "We studied the single-molecule photo-switching properties of Dronpa, a green photo-switchable fluorescent protein and a popular marker for photoactivated localization microscopy. We found the excitation light photoactivates as well as deactivates Dronpa single molecules, hindering temporal separation and limiting super resolution. To resolve this limitation, we have developed a slow-switching Dronpa variant, rsKame, featuring a V157L amino acid substitution proximal to the chromophore. The increased steric hindrance generated by the substitution reduced the excitation light-induced photoactivation from the dark to fluorescent state. To demonstrate applicability, we paired rsKame with PAmCherry1 in a two-color photoactivated localization microscopy imaging method to observe the inner and outer mitochondrial membrane structures and selectively labeled dynamin related protein 1 (Drp1), responsible for membrane scission during mitochondrial fission. We determined the diameter and length of Drp1 helical rings encircling mitochondria during fission and showed that, whereas their lengths along mitochondria were not significantly changed, their diameters decreased significantly. These results suggest support for the twistase model of Drp1 constriction, with potential loss of subunits at the helical ends." @default.
- W2079394992 created "2016-06-24" @default.
- W2079394992 creator A5002739553 @default.
- W2079394992 creator A5010743284 @default.
- W2079394992 creator A5020704012 @default.
- W2079394992 creator A5024121654 @default.
- W2079394992 creator A5051578105 @default.
- W2079394992 creator A5082768249 @default.
- W2079394992 date "2014-08-22" @default.
- W2079394992 modified "2023-10-11" @default.
- W2079394992 title "Optimized two-color super resolution imaging of Drp1 during mitochondrial fission with a slow-switching Dronpa variant" @default.
- W2079394992 cites W1657499324 @default.
- W2079394992 cites W1972145831 @default.
- W2079394992 cites W1977771229 @default.
- W2079394992 cites W1981049765 @default.
- W2079394992 cites W1981431290 @default.
- W2079394992 cites W1992858116 @default.
- W2079394992 cites W2003380824 @default.
- W2079394992 cites W2004939344 @default.
- W2079394992 cites W2007706445 @default.
- W2079394992 cites W2030383182 @default.
- W2079394992 cites W2039190163 @default.
- W2079394992 cites W2045006795 @default.
- W2079394992 cites W2048730719 @default.
- W2079394992 cites W2050722665 @default.
- W2079394992 cites W2054455698 @default.
- W2079394992 cites W2064777616 @default.
- W2079394992 cites W2072087702 @default.
- W2079394992 cites W2072904608 @default.
- W2079394992 cites W2077889241 @default.
- W2079394992 cites W2089745656 @default.
- W2079394992 cites W2094462220 @default.
- W2079394992 cites W2098181117 @default.
- W2079394992 cites W2098410588 @default.
- W2079394992 cites W2101051879 @default.
- W2079394992 cites W2110094987 @default.
- W2079394992 cites W2113550680 @default.
- W2079394992 cites W2124540225 @default.
- W2079394992 cites W2125080444 @default.
- W2079394992 cites W2126624770 @default.
- W2079394992 cites W2129769231 @default.
- W2079394992 cites W2136864660 @default.
- W2079394992 cites W2136937485 @default.
- W2079394992 cites W2139414608 @default.
- W2079394992 cites W2139868016 @default.
- W2079394992 cites W2149108521 @default.
- W2079394992 cites W2155643523 @default.
- W2079394992 cites W2156754173 @default.
- W2079394992 cites W2161892574 @default.
- W2079394992 cites W2166474296 @default.
- W2079394992 cites W2412176163 @default.
- W2079394992 doi "https://doi.org/10.1073/pnas.1320044111" @default.
- W2079394992 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4246937" @default.
- W2079394992 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25149858" @default.
- W2079394992 hasPublicationYear "2014" @default.
- W2079394992 type Work @default.
- W2079394992 sameAs 2079394992 @default.
- W2079394992 citedByCount "59" @default.
- W2079394992 countsByYear W20793949922015 @default.
- W2079394992 countsByYear W20793949922016 @default.
- W2079394992 countsByYear W20793949922017 @default.
- W2079394992 countsByYear W20793949922018 @default.
- W2079394992 countsByYear W20793949922019 @default.
- W2079394992 countsByYear W20793949922020 @default.
- W2079394992 countsByYear W20793949922021 @default.
- W2079394992 countsByYear W20793949922022 @default.
- W2079394992 countsByYear W20793949922023 @default.
- W2079394992 crossrefType "journal-article" @default.
- W2079394992 hasAuthorship W2079394992A5002739553 @default.
- W2079394992 hasAuthorship W2079394992A5010743284 @default.
- W2079394992 hasAuthorship W2079394992A5020704012 @default.
- W2079394992 hasAuthorship W2079394992A5024121654 @default.
- W2079394992 hasAuthorship W2079394992A5051578105 @default.
- W2079394992 hasAuthorship W2079394992A5082768249 @default.
- W2079394992 hasBestOaLocation W20793949921 @default.
- W2079394992 hasConcept C120665830 @default.
- W2079394992 hasConcept C121332964 @default.
- W2079394992 hasConcept C12294094 @default.
- W2079394992 hasConcept C12554922 @default.
- W2079394992 hasConcept C147080431 @default.
- W2079394992 hasConcept C152568617 @default.
- W2079394992 hasConcept C17619807 @default.
- W2079394992 hasConcept C185592680 @default.
- W2079394992 hasConcept C192468462 @default.
- W2079394992 hasConcept C28859421 @default.
- W2079394992 hasConcept C55493867 @default.
- W2079394992 hasConcept C62520636 @default.
- W2079394992 hasConcept C75473681 @default.
- W2079394992 hasConcept C86803240 @default.
- W2079394992 hasConcept C91881484 @default.
- W2079394992 hasConceptScore W2079394992C120665830 @default.
- W2079394992 hasConceptScore W2079394992C121332964 @default.
- W2079394992 hasConceptScore W2079394992C12294094 @default.
- W2079394992 hasConceptScore W2079394992C12554922 @default.
- W2079394992 hasConceptScore W2079394992C147080431 @default.
- W2079394992 hasConceptScore W2079394992C152568617 @default.
- W2079394992 hasConceptScore W2079394992C17619807 @default.
- W2079394992 hasConceptScore W2079394992C185592680 @default.