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- W2079402432 abstract "An experimental system is described for the simultaneous measurement of components of the proton motive force and other energy-related activies in microorganisms under steady defined microaerobic conditions. Oxygen is supplied in a solution containing an O2-carrier such as myoglobin or leghaemoglobin, to a stirred reaction chamber in which a suspension of the microorganism is required aboce a membrane filter whic is pervious to the carrier. The rate of O2 consumption is regulated by the rate at which the solution is pumped through the chamber. The concentration of free O2 in the chamber and the rate of its consumption are calculated from the pumping rate and the decline in the relative oxygenation of the carrier measured spectrophotometrically in the effluent solution. The uptake by the microorganisms of radioactively labelled probes for ΔpH and Δψ is calculated following their injection into the reaction chamber and monitoring of continuously collected fractions of effluent solution, after it has passed through the spectrophometer. An example of the use of the system is given. The use of this system is advocated for many microaerobic applications since most of the measurements can be made without perturbing the steady state until the final shape of the suspension is collected." @default.
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- W2079402432 date "1985-06-01" @default.
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- W2079402432 title "Measurement of components of proton motive force and related parameters in steady, microaerobic conditions" @default.
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- W2079402432 doi "https://doi.org/10.1016/0167-7012(85)90003-x" @default.
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