Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079414188> ?p ?o ?g. }
- W2079414188 endingPage "280" @default.
- W2079414188 startingPage "270" @default.
- W2079414188 abstract "Paracoccidioides brasiliensis PbP27 gene encodes a protein localized in both the fungal cytoplasm and cell wall. The parasitic infectious form produces this protein preferentially with the gene's expression varying between the fungus phylogenetic species. The biological function of the native p27 has yet to be determined during either growth of the yeast or host infection. Therefore, in this study, through the use of antisense RNA technology and Agrobacterium tumefaciens-mediated transformation, we generated mitotically stable PbP27 mutants (PbP27 aRNA) with the goal to evaluate the role of p27 in the biology and virulence of this fungus. PbP27 expression was reduced 60-75% in mutants, as determined by real-time PCR in correlation with a decrease in p27 expression. No alterations in the growth curve or in the ability to shift from mycelia to yeast or from yeast to mycelia were observed in PbP27 aRNA strains; however, we did observe a reduction in cell vitality. Moreover, a decrease in cell viability of PbP27 aRNA yeast cells after interaction with IFN-γ-stimulated macrophages was detected. Based on these results, we propose that p27 plays a role in yeast cell architecture and represents one of the mechanisms employed by this fungus for its interaction with the monocyte/macrophage system." @default.
- W2079414188 created "2016-06-24" @default.
- W2079414188 creator A5012632537 @default.
- W2079414188 creator A5014063568 @default.
- W2079414188 creator A5015479417 @default.
- W2079414188 creator A5032370238 @default.
- W2079414188 creator A5070533934 @default.
- W2079414188 creator A5077977565 @default.
- W2079414188 creator A5078271108 @default.
- W2079414188 creator A5078297851 @default.
- W2079414188 date "2013-11-01" @default.
- W2079414188 modified "2023-10-12" @default.
- W2079414188 title "<i>Paracoccidioides brasiliensis PbP27</i>gene: knockdown procedures and functional characterization" @default.
- W2079414188 cites W1578549591 @default.
- W2079414188 cites W1891793511 @default.
- W2079414188 cites W1955881849 @default.
- W2079414188 cites W1969608493 @default.
- W2079414188 cites W1977083884 @default.
- W2079414188 cites W1983381452 @default.
- W2079414188 cites W1992901210 @default.
- W2079414188 cites W1999225095 @default.
- W2079414188 cites W2008140597 @default.
- W2079414188 cites W2026554409 @default.
- W2079414188 cites W2034923231 @default.
- W2079414188 cites W2038421207 @default.
- W2079414188 cites W2046060933 @default.
- W2079414188 cites W2047407106 @default.
- W2079414188 cites W2054020573 @default.
- W2079414188 cites W2063120594 @default.
- W2079414188 cites W2066077020 @default.
- W2079414188 cites W2075602290 @default.
- W2079414188 cites W2079054135 @default.
- W2079414188 cites W2085719706 @default.
- W2079414188 cites W2100350770 @default.
- W2079414188 cites W2100837269 @default.
- W2079414188 cites W2101108802 @default.
- W2079414188 cites W2107277218 @default.
- W2079414188 cites W2107542702 @default.
- W2079414188 cites W2122283012 @default.
- W2079414188 cites W2122779464 @default.
- W2079414188 cites W2123464828 @default.
- W2079414188 cites W2130125263 @default.
- W2079414188 cites W2130943954 @default.
- W2079414188 cites W2134270883 @default.
- W2079414188 cites W2140882673 @default.
- W2079414188 cites W2144342903 @default.
- W2079414188 cites W2146538820 @default.
- W2079414188 cites W2150129353 @default.
- W2079414188 cites W2156638609 @default.
- W2079414188 cites W2160085634 @default.
- W2079414188 cites W2171964031 @default.
- W2079414188 cites W2184566907 @default.
- W2079414188 cites W2244240891 @default.
- W2079414188 cites W4233554622 @default.
- W2079414188 cites W4293247451 @default.
- W2079414188 doi "https://doi.org/10.1111/1567-1364.12099" @default.
- W2079414188 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24118983" @default.
- W2079414188 hasPublicationYear "2013" @default.
- W2079414188 type Work @default.
- W2079414188 sameAs 2079414188 @default.
- W2079414188 citedByCount "17" @default.
- W2079414188 countsByYear W20794141882014 @default.
- W2079414188 countsByYear W20794141882015 @default.
- W2079414188 countsByYear W20794141882016 @default.
- W2079414188 countsByYear W20794141882017 @default.
- W2079414188 countsByYear W20794141882018 @default.
- W2079414188 countsByYear W20794141882019 @default.
- W2079414188 countsByYear W20794141882020 @default.
- W2079414188 countsByYear W20794141882021 @default.
- W2079414188 countsByYear W20794141882023 @default.
- W2079414188 crossrefType "journal-article" @default.
- W2079414188 hasAuthorship W2079414188A5012632537 @default.
- W2079414188 hasAuthorship W2079414188A5014063568 @default.
- W2079414188 hasAuthorship W2079414188A5015479417 @default.
- W2079414188 hasAuthorship W2079414188A5032370238 @default.
- W2079414188 hasAuthorship W2079414188A5070533934 @default.
- W2079414188 hasAuthorship W2079414188A5077977565 @default.
- W2079414188 hasAuthorship W2079414188A5078271108 @default.
- W2079414188 hasAuthorship W2079414188A5078297851 @default.
- W2079414188 hasBestOaLocation W20794141881 @default.
- W2079414188 hasConcept C104317684 @default.
- W2079414188 hasConcept C143065580 @default.
- W2079414188 hasConcept C150194340 @default.
- W2079414188 hasConcept C173396325 @default.
- W2079414188 hasConcept C204241405 @default.
- W2079414188 hasConcept C2775839086 @default.
- W2079414188 hasConcept C2776162087 @default.
- W2079414188 hasConcept C2777576037 @default.
- W2079414188 hasConcept C2777681368 @default.
- W2079414188 hasConcept C2779108831 @default.
- W2079414188 hasConcept C2779222958 @default.
- W2079414188 hasConcept C2909986799 @default.
- W2079414188 hasConcept C54355233 @default.
- W2079414188 hasConcept C60987743 @default.
- W2079414188 hasConcept C86803240 @default.
- W2079414188 hasConcept C89423630 @default.
- W2079414188 hasConcept C95444343 @default.
- W2079414188 hasConceptScore W2079414188C104317684 @default.