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• W2079418166 abstract "Cardiac damages caused by in vivo infection with Trypanosoma cruzi are still not fully clarified. Here we describe for the first time an in vitro model of fibrosis, hypertrophy, and remodeling induced by T. cruzi in cardiomyocyte spheroids (cardiac microtissues). In this new 3-dimensional system, cardiac spheroids showed spontaneous contractility, with typical cardiac morphology and production of extracellular matrix components. There were 4-and 6-fold increases, respectively, in the area and the volume of T. cruzi -infected cardiomyocytes and whole micro-tissues, together with a 50% reduction of the cell population. Immunofluorescence showed increased expression of fibronectin, collagen IV, and laminin in the microtissues 144 h after infection. T. cruzi infection induced an increaseinboththecellularareaandtheextracellularmatrixcomponentsincardiacspheroids,whichcontributed to an increase in total microtissue volume, making this a powerful 3-dimensional in vitro model for the study of cardiac-tissue hypertrophy, fibrosis, and remodeling" @default.
• W2079418166 created "2016-06-24" @default.
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• W2079418166 date "2008-03-15" @default.
• W2079418166 modified "2023-10-01" @default.
• W2079418166 title "Fibrosis and Hypertrophy Induced by<i>Trypanosoma cruzi</i>in a Three‐Dimensional Cardiomyocyte‐Culture System" @default.
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• W2079418166 doi "https://doi.org/10.1086/528373" @default.
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