Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079423776> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2079423776 endingPage "S92" @default.
- W2079423776 startingPage "S92" @default.
- W2079423776 abstract "342 Introduction: Pancreas from non-heartbeating cadaver can be available in pancreas islet transplantation if its damage from warm ischamia (WI) could be reduced by a suitable method. The two-layer cold storage method (TL), which utilizes perfluorochemical and UW solution, has previously demonstrated to have unique characteristics of supplying oxygen to the graft, effectively maintaining the viability of whole pancreas grafts. In this study we evaluated the effect of TL on islet yield and viability of WI damaged pancreas. Method: Dog pancreases were divided into following five groups (group 1; n=10, groups 2∼5; n=5). In group 1, pancreases were processed immediately after total pancreatectomy. In groups 2 (4), pancreases were left in the abdominal cavity for 60 (90) minutes as WI, then processed into islets. In groups 3 (5), pancreases were left in the abdominal cavity for 60 (90) minutes as WI followed by TL for 24 hours at 4°C, then processed into islets. Islet isolation was performed by the automated collagenase digestion method of Ricordi, purified on Euro-Ficoll discontinuous gradients. We compared five groups in terms of islet yield, expressed as islet equivalents (IEQ, diameter standardizing to 150 μm) per pancreas weight (IEQ/g pancreas). Results: Islet yield was 2700 ± 1200 (mean±SD) in group 1, 1500 ± 700 in group 2, (p=0.03 vs. group 1, p=0.02 vs. group 3), and 2600 ± 600 in group 3 (p=0.80 vs. group 1). In group 4 and 5, almost no islets were obtained after purification. In static incubation islets in group 1 and 3 showed sufficient insulin release in response to glucose stimuli and this means that viability of islets in group 1 and 3 was maintained. Conclusions: These data clearly demonstrates that TL following WI can regain islet yield and viability almost at the same level as those of fresh pancreas. So it could be possible to isolate islets from pancreas harvested from non-heartbeating cadaver by TL." @default.
- W2079423776 created "2016-06-24" @default.
- W2079423776 creator A5008002384 @default.
- W2079423776 creator A5009329552 @default.
- W2079423776 creator A5018975124 @default.
- W2079423776 creator A5041602945 @default.
- W2079423776 creator A5046238727 @default.
- W2079423776 creator A5051761667 @default.
- W2079423776 creator A5058652786 @default.
- W2079423776 creator A5078799766 @default.
- W2079423776 creator A5078918448 @default.
- W2079423776 creator A5081530778 @default.
- W2079423776 date "1999-04-01" @default.
- W2079423776 modified "2023-09-26" @default.
- W2079423776 title "THE POSSIBILITY OF ISLET ISOLATION FROM PANCREAS HARVESTED FROM NON-HEART BEATING CADAVER" @default.
- W2079423776 doi "https://doi.org/10.1097/00007890-199904150-00370" @default.
- W2079423776 hasPublicationYear "1999" @default.
- W2079423776 type Work @default.
- W2079423776 sameAs 2079423776 @default.
- W2079423776 citedByCount "0" @default.
- W2079423776 crossrefType "journal-article" @default.
- W2079423776 hasAuthorship W2079423776A5008002384 @default.
- W2079423776 hasAuthorship W2079423776A5009329552 @default.
- W2079423776 hasAuthorship W2079423776A5018975124 @default.
- W2079423776 hasAuthorship W2079423776A5041602945 @default.
- W2079423776 hasAuthorship W2079423776A5046238727 @default.
- W2079423776 hasAuthorship W2079423776A5051761667 @default.
- W2079423776 hasAuthorship W2079423776A5058652786 @default.
- W2079423776 hasAuthorship W2079423776A5078799766 @default.
- W2079423776 hasAuthorship W2079423776A5078918448 @default.
- W2079423776 hasAuthorship W2079423776A5081530778 @default.
- W2079423776 hasBestOaLocation W20794237761 @default.
- W2079423776 hasConcept C105702510 @default.
- W2079423776 hasConcept C113278225 @default.
- W2079423776 hasConcept C126322002 @default.
- W2079423776 hasConcept C126894567 @default.
- W2079423776 hasConcept C165220095 @default.
- W2079423776 hasConcept C16685009 @default.
- W2079423776 hasConcept C181199279 @default.
- W2079423776 hasConcept C185592680 @default.
- W2079423776 hasConcept C2778394429 @default.
- W2079423776 hasConcept C2778764654 @default.
- W2079423776 hasConcept C2779306644 @default.
- W2079423776 hasConcept C2780936613 @default.
- W2079423776 hasConcept C2911091166 @default.
- W2079423776 hasConcept C55493867 @default.
- W2079423776 hasConcept C71924100 @default.
- W2079423776 hasConceptScore W2079423776C105702510 @default.
- W2079423776 hasConceptScore W2079423776C113278225 @default.
- W2079423776 hasConceptScore W2079423776C126322002 @default.
- W2079423776 hasConceptScore W2079423776C126894567 @default.
- W2079423776 hasConceptScore W2079423776C165220095 @default.
- W2079423776 hasConceptScore W2079423776C16685009 @default.
- W2079423776 hasConceptScore W2079423776C181199279 @default.
- W2079423776 hasConceptScore W2079423776C185592680 @default.
- W2079423776 hasConceptScore W2079423776C2778394429 @default.
- W2079423776 hasConceptScore W2079423776C2778764654 @default.
- W2079423776 hasConceptScore W2079423776C2779306644 @default.
- W2079423776 hasConceptScore W2079423776C2780936613 @default.
- W2079423776 hasConceptScore W2079423776C2911091166 @default.
- W2079423776 hasConceptScore W2079423776C55493867 @default.
- W2079423776 hasConceptScore W2079423776C71924100 @default.
- W2079423776 hasIssue "7" @default.
- W2079423776 hasLocation W20794237761 @default.
- W2079423776 hasLocation W20794237762 @default.
- W2079423776 hasOpenAccess W2079423776 @default.
- W2079423776 hasPrimaryLocation W20794237761 @default.
- W2079423776 hasRelatedWork W1976415797 @default.
- W2079423776 hasRelatedWork W2028788305 @default.
- W2079423776 hasRelatedWork W2029307475 @default.
- W2079423776 hasRelatedWork W2058657286 @default.
- W2079423776 hasRelatedWork W2062642389 @default.
- W2079423776 hasRelatedWork W2108606670 @default.
- W2079423776 hasRelatedWork W2321169792 @default.
- W2079423776 hasRelatedWork W2396530875 @default.
- W2079423776 hasRelatedWork W2398078892 @default.
- W2079423776 hasRelatedWork W2419194992 @default.
- W2079423776 hasVolume "67" @default.
- W2079423776 isParatext "false" @default.
- W2079423776 isRetracted "false" @default.
- W2079423776 magId "2079423776" @default.
- W2079423776 workType "article" @default.