SemOpenAlex |

SemOpenAlex

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079425449> ?p ?o ?g. }

Showing items 1 to 100 of ±123 with 100 items per page.

W2079425449 endingPage "829" @default.
W2079425449 startingPage "821" @default.
W2079425449 abstract "T4 must be activated by its monodeiodination to T3 by type 1 or 2 iodothyronine deiodinase (D1 and D2). Recent studies show that despite an approximately 2000-fold higher Michaelis constant (Km; T4) for D1 than for D2 using dithiothreitol (DTT) as cofactor, D1 expressed in intact cells produces T3 at free T4 concentrations many orders of magnitude below its Km. To understand the factors regulating D1 and D2 catalysis in vivo, we studied a mutant D2 with a proline at position 135 of the active center of D2 replaced with a serine, as found in D1. The P135S D2 enzyme has many D1-like properties, a Km (T4) in the micromolar range, ping-pong kinetics with DTT, and sensitivity to 6n-propylthiouracil (PTU) in vitro. Unexpectedly, when the P135S D2 was expressed in HEK-293 cells and exposed to 2–200 pm free T4, the rate of T4 to T3 conversion was identical with D2 and conversion was insensitive to PTU. Using glutathione as a cofactor in vitro resulted in a marked decrease in the Km (T4) (as also occurs for D1), it showed sequential kinetics with T4 and it was sensitive to PTU but was resistant when HEK-293 cytosol was used as a cofactor. Thus, the in vivo catalytic properties of the P135S D2 mutant are more accurately predicted from in vitro studies with weak reducing agents, such as glutathione or endogenous cofactors, than by those with DTT." @default.
W2079425449 created "2016-06-24" @default.
W2079425449 creator A5003076812 @default.
W2079425449 creator A5007399848 @default.
W2079425449 creator A5015835109 @default.
W2079425449 creator A5038208451 @default.
W2079425449 creator A5040787757 @default.
W2079425449 creator A5089972574 @default.
W2079425449 date "2010-02-01" @default.
W2079425449 modified "2023-10-12" @default.
W2079425449 title "Substitution of Serine for Proline in the Active Center of Type 2 Iodothyronine Deiodinase Substantially Alters Its in Vitro Biochemical Properties with Dithiothreitol But Not Its Function in Intact Cells" @default.
W2079425449 cites W1505425247 @default.
W2079425449 cites W1521890372 @default.
W2079425449 cites W1788190869 @default.
W2079425449 cites W1963784968 @default.
W2079425449 cites W1969391229 @default.
W2079425449 cites W1978715342 @default.
W2079425449 cites W1982195382 @default.
W2079425449 cites W1984810754 @default.
W2079425449 cites W1997378330 @default.
W2079425449 cites W2002027673 @default.
W2079425449 cites W2006140668 @default.
W2079425449 cites W2022624295 @default.
W2079425449 cites W2023817599 @default.
W2079425449 cites W2031239072 @default.
W2079425449 cites W2034778853 @default.
W2079425449 cites W2049634399 @default.
W2079425449 cites W2055205764 @default.
W2079425449 cites W2064598604 @default.
W2079425449 cites W2074814823 @default.
W2079425449 cites W2075650590 @default.
W2079425449 cites W2084429552 @default.
W2079425449 cites W2092277207 @default.
W2079425449 cites W2098101263 @default.
W2079425449 cites W2102809671 @default.
W2079425449 cites W2107919252 @default.
W2079425449 cites W2119053116 @default.
W2079425449 cites W2123790179 @default.
W2079425449 cites W2139309835 @default.
W2079425449 cites W2152614869 @default.
W2079425449 cites W2161750831 @default.
W2079425449 cites W2339552885 @default.
W2079425449 cites W2341939306 @default.
W2079425449 doi "https://doi.org/10.1210/en.2009-0980" @default.
W2079425449 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2817617" @default.
W2079425449 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19966180" @default.
W2079425449 hasPublicationYear "2010" @default.
W2079425449 type Work @default.
W2079425449 sameAs 2079425449 @default.
W2079425449 citedByCount "16" @default.
W2079425449 countsByYear W20794254492012 @default.
W2079425449 countsByYear W20794254492013 @default.
W2079425449 countsByYear W20794254492014 @default.
W2079425449 countsByYear W20794254492015 @default.
W2079425449 countsByYear W20794254492016 @default.
W2079425449 countsByYear W20794254492022 @default.
W2079425449 countsByYear W20794254492023 @default.
W2079425449 crossrefType "journal-article" @default.
W2079425449 hasAuthorship W2079425449A5003076812 @default.
W2079425449 hasAuthorship W2079425449A5007399848 @default.
W2079425449 hasAuthorship W2079425449A5015835109 @default.
W2079425449 hasAuthorship W2079425449A5038208451 @default.
W2079425449 hasAuthorship W2079425449A5040787757 @default.
W2079425449 hasAuthorship W2079425449A5089972574 @default.
W2079425449 hasBestOaLocation W20794254491 @default.
W2079425449 hasConcept C126322002 @default.
W2079425449 hasConcept C134018914 @default.
W2079425449 hasConcept C150903083 @default.
W2079425449 hasConcept C181199279 @default.
W2079425449 hasConcept C185592680 @default.
W2079425449 hasConcept C197957613 @default.
W2079425449 hasConcept C207001950 @default.
W2079425449 hasConcept C2775960720 @default.
W2079425449 hasConcept C2776414213 @default.
W2079425449 hasConcept C2777824588 @default.
W2079425449 hasConcept C2778288131 @default.
W2079425449 hasConcept C2778417266 @default.
W2079425449 hasConcept C538909803 @default.
W2079425449 hasConcept C55493867 @default.
W2079425449 hasConcept C71315377 @default.
W2079425449 hasConcept C71924100 @default.
W2079425449 hasConcept C86803240 @default.
W2079425449 hasConceptScore W2079425449C126322002 @default.
W2079425449 hasConceptScore W2079425449C134018914 @default.
W2079425449 hasConceptScore W2079425449C150903083 @default.
W2079425449 hasConceptScore W2079425449C181199279 @default.
W2079425449 hasConceptScore W2079425449C185592680 @default.
W2079425449 hasConceptScore W2079425449C197957613 @default.
W2079425449 hasConceptScore W2079425449C207001950 @default.
W2079425449 hasConceptScore W2079425449C2775960720 @default.
W2079425449 hasConceptScore W2079425449C2776414213 @default.
W2079425449 hasConceptScore W2079425449C2777824588 @default.
W2079425449 hasConceptScore W2079425449C2778288131 @default.
W2079425449 hasConceptScore W2079425449C2778417266 @default.
W2079425449 hasConceptScore W2079425449C538909803 @default.
W2079425449 hasConceptScore W2079425449C55493867 @default.
W2079425449 hasConceptScore W2079425449C71315377 @default.
W2079425449 hasConceptScore W2079425449C71924100 @default.