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- W2079435132 abstract "The discovery and characterization of microRNAs (miRNAs) and other families of short RNAs has led to a rapid expansion of research directed at elucidating their expression patterns and regulatory functions. Here, we describe a convenient, sensitive, and straightforward method to detect and quantitate specific miRNA levels in unfractionated total RNA samples. The method, based on splinted ligation, does not require specialized equipment or any amplification step, and is significantly faster and more sensitive than Northern blotting. We demonstrate that the method can be used to detect various classes of small regulatory RNAs from different organisms." @default.
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- W2079435132 date "2007-04-24" @default.
- W2079435132 modified "2023-10-12" @default.
- W2079435132 title "A rapid, quantitative assay for direct detection of microRNAs and other small RNAs using splinted ligation" @default.
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- W2079435132 doi "https://doi.org/10.1261/rna.518107" @default.
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