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- W2079437004 abstract "Photometric measurements of DNA in Acheta domesticus were made on single nuclei and on each pachytene chromosome, chromomere by chromomere. The extinction values were processed by a computer. The background is negligible and there is good agreement between the chromomere pattern and the amount of DNA found. The 2C value of Acheta brain nuclei is 4.0 pg. The following DNA values were obtained for oocyte nuclei at early pachytene. (1) All DNA bodies or major chromomeres 4.18 pg. (2) The chromosomes only, 9.11 pg. (3) The amplified major chromomere of chromosome 6, 2.00 pg. (4) The major chromomere of chromosome 11, 1.48 pg. The 11 pachytene chromosomes of the oocytes contain 0.4, 0.3, 0.3, 0.2, 0.2, 0.2, 0.1, 0.1, 0.1, 0.1 and 0.1 X 109 nucleotide pairs respectively (haploid value). The amplicon (the site of amplification) of chromosome 6 contains 0.02 pg whereas that of chromosome 11 has 0.01 pg (haploid value); this corresponds to 0.02 X 108 and 0.01X 109 nucleotide pairs respectively. In the case of chromosome 6, the amplified major chromomere contains 1.9-2.5 X 109 nucleotide pairs, which is circa 100 times the DNA of its amplicon (haploid). An average pachytene chromomere (haploid) in Acheta has 0.01 X 109 nucleotide pairs. The location in specific chromomeres of well-defined genes such as the cistrons for 28S and 18S ribosomal RNA, coupled with knowledge of the number of nucleotide pairs in these chromomeres helps to elucidate the organization and function of the genetic units in eukaryotes." @default.
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- W2079437004 date "2009-02-12" @default.
- W2079437004 modified "2023-10-03" @default.
- W2079437004 title "Amplification of ribosomal DNA in Acheta" @default.
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- W2079437004 doi "https://doi.org/10.1111/j.1601-5223.1973.tb01073.x" @default.
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