Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079454393> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2079454393 endingPage "4564" @default.
- W2079454393 startingPage "4557" @default.
- W2079454393 abstract "The kinetic parameters of bovine prothrombin activation by factor Xa were determined in the absence and presence of factor Va as a function of the phospholipid concentration and composition. In the absence of factor Va, the Km for prothrombin increases proportionally with the phospholipid concentration and correlates well with the affinity of prothrombin for the different membranes. Phospholipid vesicles with a high affinity for prothrombin yield low Km values compared to membranes with less favorable binding parameters. At limited phospholipid concentrations, the Vmax of prothrombin activation correlates with the binding affinity of factor Xa for the various phospholipid vesicles. Membranes with a high affinity for factor Xa have high Vmax values, while for membranes with a low affinity a low Vmax is observed. Extrapolation of double-reciprocal plots of 1/Vmax vs. 1/[phospholipid] to infinite phospholipid concentrations, a condition at which all factor Xa would participate in prothrombin activation, yields a kcat of 2-4 min-1 independent of the type and amount of acidic phospholipid present in the vesicles. Also, in the presence of factor Va the Km for prothrombin varies proportionally with the phospholipid concentration. There is, however, no correlation between the binding parameters and the Km. Factor Va drastically lowers the Km for prothrombin for vesicles that have a low affinity for prothrombin. Vesicles composed of 20 mol % phosphatidylglycerol and 80 mol % phosphatidylcholine have a Km of 0.04 microM when factor Va is present, compared to 2.2 microM determined in the absence of factor Va.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2079454393 created "2016-06-24" @default.
- W2079454393 creator A5053372098 @default.
- W2079454393 creator A5059391394 @default.
- W2079454393 creator A5069031083 @default.
- W2079454393 creator A5087919219 @default.
- W2079454393 date "1984-09-25" @default.
- W2079454393 modified "2023-09-23" @default.
- W2079454393 title "Kinetic studies of prothrombin activation: effect of factor Va and phospholipids on formation of the enzyme-substrate complex" @default.
- W2079454393 cites W1484115349 @default.
- W2079454393 cites W1490507833 @default.
- W2079454393 cites W1531765158 @default.
- W2079454393 cites W1533471204 @default.
- W2079454393 cites W1921593426 @default.
- W2079454393 cites W1961597147 @default.
- W2079454393 cites W1983600681 @default.
- W2079454393 cites W1991651684 @default.
- W2079454393 cites W2011105427 @default.
- W2079454393 cites W2014279568 @default.
- W2079454393 cites W2016445477 @default.
- W2079454393 cites W2026360066 @default.
- W2079454393 cites W2038774484 @default.
- W2079454393 cites W2056110296 @default.
- W2079454393 cites W2065156100 @default.
- W2079454393 cites W2083047029 @default.
- W2079454393 cites W2091317550 @default.
- W2079454393 cites W2093239579 @default.
- W2079454393 cites W2096326108 @default.
- W2079454393 cites W2105341627 @default.
- W2079454393 cites W2126206535 @default.
- W2079454393 cites W2170055916 @default.
- W2079454393 doi "https://doi.org/10.1021/bi00315a008" @default.
- W2079454393 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6498156" @default.
- W2079454393 hasPublicationYear "1984" @default.
- W2079454393 type Work @default.
- W2079454393 sameAs 2079454393 @default.
- W2079454393 citedByCount "87" @default.
- W2079454393 countsByYear W20794543932012 @default.
- W2079454393 countsByYear W20794543932013 @default.
- W2079454393 countsByYear W20794543932015 @default.
- W2079454393 countsByYear W20794543932016 @default.
- W2079454393 countsByYear W20794543932018 @default.
- W2079454393 crossrefType "journal-article" @default.
- W2079454393 hasAuthorship W2079454393A5053372098 @default.
- W2079454393 hasAuthorship W2079454393A5059391394 @default.
- W2079454393 hasAuthorship W2079454393A5069031083 @default.
- W2079454393 hasAuthorship W2079454393A5087919219 @default.
- W2079454393 hasConcept C112516734 @default.
- W2079454393 hasConcept C12554922 @default.
- W2079454393 hasConcept C130316041 @default.
- W2079454393 hasConcept C185592680 @default.
- W2079454393 hasConcept C203014093 @default.
- W2079454393 hasConcept C2776330855 @default.
- W2079454393 hasConcept C2777292125 @default.
- W2079454393 hasConcept C2777444498 @default.
- W2079454393 hasConcept C2778918659 @default.
- W2079454393 hasConcept C41625074 @default.
- W2079454393 hasConcept C43617362 @default.
- W2079454393 hasConcept C55493867 @default.
- W2079454393 hasConcept C86803240 @default.
- W2079454393 hasConcept C89560881 @default.
- W2079454393 hasConceptScore W2079454393C112516734 @default.
- W2079454393 hasConceptScore W2079454393C12554922 @default.
- W2079454393 hasConceptScore W2079454393C130316041 @default.
- W2079454393 hasConceptScore W2079454393C185592680 @default.
- W2079454393 hasConceptScore W2079454393C203014093 @default.
- W2079454393 hasConceptScore W2079454393C2776330855 @default.
- W2079454393 hasConceptScore W2079454393C2777292125 @default.
- W2079454393 hasConceptScore W2079454393C2777444498 @default.
- W2079454393 hasConceptScore W2079454393C2778918659 @default.
- W2079454393 hasConceptScore W2079454393C41625074 @default.
- W2079454393 hasConceptScore W2079454393C43617362 @default.
- W2079454393 hasConceptScore W2079454393C55493867 @default.
- W2079454393 hasConceptScore W2079454393C86803240 @default.
- W2079454393 hasConceptScore W2079454393C89560881 @default.
- W2079454393 hasIssue "20" @default.
- W2079454393 hasLocation W20794543931 @default.
- W2079454393 hasLocation W20794543932 @default.
- W2079454393 hasOpenAccess W2079454393 @default.
- W2079454393 hasPrimaryLocation W20794543931 @default.
- W2079454393 hasRelatedWork W1977417712 @default.
- W2079454393 hasRelatedWork W1980595834 @default.
- W2079454393 hasRelatedWork W1983547075 @default.
- W2079454393 hasRelatedWork W1993816515 @default.
- W2079454393 hasRelatedWork W2027206139 @default.
- W2079454393 hasRelatedWork W2079454393 @default.
- W2079454393 hasRelatedWork W2086178247 @default.
- W2079454393 hasRelatedWork W2112490945 @default.
- W2079454393 hasRelatedWork W2562839415 @default.
- W2079454393 hasRelatedWork W2789079761 @default.
- W2079454393 hasVolume "23" @default.
- W2079454393 isParatext "false" @default.
- W2079454393 isRetracted "false" @default.
- W2079454393 magId "2079454393" @default.
- W2079454393 workType "article" @default.