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• W2079461408 endingPage "227" @default.
• W2079461408 startingPage "227" @default.
• W2079461408 abstract "Outer membrane proteins of Pasteurella (P.) multocida have been known to be protective immunogens. Pasteurella lipoprotein E (PlpE) has been reported to be an important cross reactive outer membrane protein in P. multocida. The gene encoding the PlpE of P. multocida serotypes A: 3, B: 2 and D: 1 was amplified from the genomic DNA. The amplified products were cloned and the nucleotide sequence was determined. Sequence analysis of the recombinant clones revealed a single open reading frame of 1,011 bp, 1,008 bp and 1,017 bp encoding a protein with a calculated molecular mass of 37.829 kDa, 37.389 kDa and 37.965 kDa for serotypes A: 3, B: 2 and D: 1 respectively. The comparison of the plpE sequence in different capsular types revealed a high degree (> 90%) of homology. Furthermore, the plpE gene of Haemorhhagic septicaemia causing serotype (B: 2) was expressed in E. coli and recombinant PlpE was strongly immunostained by antiserum against whole cell antigen, indicating that the protein is expressed in vivo." @default.
• W2079461408 created "2016-06-24" @default.
• W2079461408 creator A5001124468 @default.
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• W2079461408 date "2010-01-01" @default.
• W2079461408 modified "2023-10-10" @default.
• W2079461408 title "Molecular heterogeneity of<i>plp</i>E gene in Indian isolates of<i>Pasteurella multocida</i>and expression of recombinant PlpE in vaccine strain of<i>P. multocida</i>serotype B: 2" @default.
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• W2079461408 doi "https://doi.org/10.4142/jvs.2010.11.3.227" @default.
• W2079461408 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2924484" @default.
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