Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079463072> ?p ?o ?g. }
- W2079463072 endingPage "11547" @default.
- W2079463072 startingPage "11539" @default.
- W2079463072 abstract "The pH dependence of the kinetic parameters for the reaction catalyzed by NADPH-cytochrome P-450 oxidoreductase (P-450R) has been determined, using various substrates and inhibitors. All Vmax and (V/K) profiles show pKas of 6.2-7.3, for an acidic group that is preferentially unprotonated for catalysis, and of 8.1-9.6, for a basic group that is preferentially protonated for catalysis. The presence of the wrong ionization state for both of these groups is tolerated more at lower ionic strength (300 mM) than at higher ionic strength (850 mM). Ionization of the basic group has a more pronounced effect on binding of substrate (cytochrome c or dichloroindophenol) than on catalysis, since ionization has only a 2-fold effect on Vmax with cytochrome c, and only a 5-fold effect on Vmax with dichloroindophenol, while (V/K) for both substrates continues to drop at high pH with no sign of reaching a plateau. Therefore, this basic group affects predominantly substrate binding and, to a lesser extent, catalysis. It is most likely located on the surface of the protein at the cytochrome c/dichloroindophenol binding site, near the FMN prosthetic group. The NADP+ pKi profile shows a pKa of 5.95 for the 2'-phosphate of NADP+, which is bound to P-450R as the dianion, and a pKa of 9.53 for an enzyme group that must be protonated in order to bind NADP+.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W2079463072 created "2016-06-24" @default.
- W2079463072 creator A5055937343 @default.
- W2079463072 creator A5089397767 @default.
- W2079463072 date "1993-01-26" @default.
- W2079463072 modified "2023-09-26" @default.
- W2079463072 title "Enzyme-substrate binding interactions of NADPH-cytochrome P-450 oxidoreductase characterized with pH and alternate substrate/inhibitor studies" @default.
- W2079463072 cites W1248348299 @default.
- W2079463072 cites W126145571 @default.
- W2079463072 cites W1487187329 @default.
- W2079463072 cites W1488511002 @default.
- W2079463072 cites W1499452131 @default.
- W2079463072 cites W1523760158 @default.
- W2079463072 cites W1546744738 @default.
- W2079463072 cites W1553966958 @default.
- W2079463072 cites W1554826669 @default.
- W2079463072 cites W1578345157 @default.
- W2079463072 cites W1579297624 @default.
- W2079463072 cites W1579514744 @default.
- W2079463072 cites W1584574994 @default.
- W2079463072 cites W1589756962 @default.
- W2079463072 cites W1596788321 @default.
- W2079463072 cites W1600839384 @default.
- W2079463072 cites W1603947018 @default.
- W2079463072 cites W1606791548 @default.
- W2079463072 cites W1638893987 @default.
- W2079463072 cites W1639043875 @default.
- W2079463072 cites W1775749144 @default.
- W2079463072 cites W1969802703 @default.
- W2079463072 cites W1971758819 @default.
- W2079463072 cites W1972984319 @default.
- W2079463072 cites W1979034187 @default.
- W2079463072 cites W1982857351 @default.
- W2079463072 cites W1991726159 @default.
- W2079463072 cites W2006352258 @default.
- W2079463072 cites W2006369456 @default.
- W2079463072 cites W2009515393 @default.
- W2079463072 cites W2009752716 @default.
- W2079463072 cites W2012135403 @default.
- W2079463072 cites W2014704008 @default.
- W2079463072 cites W2015115055 @default.
- W2079463072 cites W2016281849 @default.
- W2079463072 cites W2022798135 @default.
- W2079463072 cites W2041368185 @default.
- W2079463072 cites W2047850186 @default.
- W2079463072 cites W2048982417 @default.
- W2079463072 cites W2049177783 @default.
- W2079463072 cites W2050246593 @default.
- W2079463072 cites W2055724834 @default.
- W2079463072 cites W2057317771 @default.
- W2079463072 cites W2058482841 @default.
- W2079463072 cites W2062254955 @default.
- W2079463072 cites W2065475310 @default.
- W2079463072 cites W2067489100 @default.
- W2079463072 cites W2076876823 @default.
- W2079463072 cites W2081082999 @default.
- W2079463072 cites W2084142095 @default.
- W2079463072 cites W2087908239 @default.
- W2079463072 cites W2088516681 @default.
- W2079463072 cites W2200991233 @default.
- W2079463072 cites W2266328638 @default.
- W2079463072 cites W2411847774 @default.
- W2079463072 cites W2969273703 @default.
- W2079463072 cites W3118710269 @default.
- W2079463072 cites W4889063 @default.
- W2079463072 doi "https://doi.org/10.1021/bi00094a010" @default.
- W2079463072 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8218221" @default.
- W2079463072 hasPublicationYear "1993" @default.
- W2079463072 type Work @default.
- W2079463072 sameAs 2079463072 @default.
- W2079463072 citedByCount "24" @default.
- W2079463072 countsByYear W20794630722014 @default.
- W2079463072 countsByYear W20794630722015 @default.
- W2079463072 countsByYear W20794630722017 @default.
- W2079463072 countsByYear W20794630722018 @default.
- W2079463072 countsByYear W20794630722021 @default.
- W2079463072 crossrefType "journal-article" @default.
- W2079463072 hasAuthorship W2079463072A5055937343 @default.
- W2079463072 hasAuthorship W2079463072A5089397767 @default.
- W2079463072 hasConcept C111368507 @default.
- W2079463072 hasConcept C127313418 @default.
- W2079463072 hasConcept C145148216 @default.
- W2079463072 hasConcept C161790260 @default.
- W2079463072 hasConcept C178790620 @default.
- W2079463072 hasConcept C181199279 @default.
- W2079463072 hasConcept C184651966 @default.
- W2079463072 hasConcept C185592680 @default.
- W2079463072 hasConcept C187428577 @default.
- W2079463072 hasConcept C2777289219 @default.
- W2079463072 hasConcept C2779268744 @default.
- W2079463072 hasConcept C2780768313 @default.
- W2079463072 hasConcept C28859421 @default.
- W2079463072 hasConcept C29311851 @default.
- W2079463072 hasConcept C30095370 @default.
- W2079463072 hasConcept C41183919 @default.
- W2079463072 hasConcept C55493867 @default.
- W2079463072 hasConcept C56856141 @default.
- W2079463072 hasConcept C71240020 @default.