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- W2079466882 abstract "1.1. A method has been described to localized the alkaline phosphomonoesterase in paraffin sections. The original method of Gomori was used with various modifications however.2.2. It is shown that a diffuse picture is obtained when the sections placed on a slide are brought into water via ethanol. This picture is formed by the solubility of the enzyme in diluted ethanol. It is recommended to execute this reaction with non-deparaffined sections.3.3. It appears from control experiments where the substrate has been replaced by Ca(NO3)2 that at pH=9.4 and higher a sediment of unsoluble Ca-compounds appears in the sections which have not been formed by the action of phosphatase. This formation has not been obtained at pH=8.4. If one would like to execute this reaction at pH=9.4 then it is necessary to wash out the sections with distilled water first.4.4. If the reaction proceeds at pH=8.4 no blackening is to be perceived in sections in which the enzyme has been destroyed before the reaction either by heating or by u.v. light. This proves that the blackening which otherwise appears at pH=8.4 must in fact be ascribed to the action of the phosphatase.At pH=9.4 a blackening has been formed in sections that have previously been heated with or without substrate. It is identical with that in the control sections (mentioned under 3).5.5. From control experiments it has appeared that in the sections the blackening of the nuclei is not due to the phosphatase activity. In an extensive discussion it is pointed out that we may not conclude from the histochemical research that nuclei contain alkaline phosphatase." @default.
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- W2079466882 date "1949-01-01" @default.
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- W2079466882 title "A critical examination of the histochemical demonstration of the alkaline phosphomonoesterase" @default.
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- W2079466882 doi "https://doi.org/10.1016/0006-3002(49)90084-0" @default.
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