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- W2079477727 abstract "Abstract In the presence of purified nitrate reductase (NR) and 1 mM NADH, illuminated pea chloroplasts catalysed reduction of NO 3 − to NH 3 with the concomitant evolution of O 2 . The rates were slightly less than those for reduction of NO 2 − to NH 3 and O 2 , evolution by chloroplasts in the absence of NR and NADH ( ca 6 μg atoms N/mg Chl/hr). Illuminated chloroplasts quantitatively reduced 0.2 mM oxaloacetate (OAA) to malate. In the presence of an extrachloroplast malate-oxidizing system comprised of NAD-specific malate dehydrogenase (NAD-MDH), NAD, NR and NO 3 − , illuminated chloroplasts supported OAA-dependent reduction of NO 3 − to NH 3 with the evolution of O 2 . The reaction did not proceed in the absence of any of these supplements or in the dark but malate could replace OAA. The results are consistent with the reduction of NO 3 − by reducing equivalents from H 2 O involving a malate/OAA shuttle. The ratios for O 2 , evolved: C 4 -acid supplied and N reduced: C 4 -acid supplied in certain experiments imply recycling of the C 4 -acids." @default.
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- W2079477727 date "1980-01-01" @default.
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- W2079477727 title "Light dependent reduction of nitrate by pea chloroplasts in the presence of nitrate reductase and C4-dicarboxylic acids" @default.
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- W2079477727 doi "https://doi.org/10.1016/0031-9422(80)83005-6" @default.
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