Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079478351> ?p ?o ?g. }
- W2079478351 abstract "This work was undertaken to investigate the activation of pig oocytes after microinjection of crude sperm extract prepared from ejaculated boar spermatozoa. The results were compared with those of electro-stimulated oocytes. When in vitro-matured pig oocytes were microinjected with sperm extract and others were electro-stimulated, 100% and 92%, respectively, of the oocytes were released from arrest at metaphase II (MII) and formed female pronuclei. To test their developmental ability, the injected oocytes were treated with cytochalasin B and then cultured in NCSU23 medium. After 168 h, 30% and 44% of the oocytes that had been microinjected with sperm extract and electro-stimulated, respectively, developed to the blastocyst stage. At 6-8 h after the microinjection of sperm extract or electro-stimulation, cortical granules were released from the oocytes. In addition, Cdc2 kinase activity declined to a low level in the treated oocytes. These results indicate that microinjection of crude sperm extract induces the release of in vitro-matured pig oocytes from MII-arrest and leads them into a series of events related to oocyte activation." @default.
- W2079478351 created "2016-06-24" @default.
- W2079478351 creator A5009730530 @default.
- W2079478351 creator A5054132178 @default.
- W2079478351 creator A5085907353 @default.
- W2079478351 date "2004-01-01" @default.
- W2079478351 modified "2023-09-24" @default.
- W2079478351 title "Activation and Development of Pig Oocytes after Microinjection of Crude Sperm Extract" @default.
- W2079478351 cites W1434994921 @default.
- W2079478351 cites W1736541167 @default.
- W2079478351 cites W1801735381 @default.
- W2079478351 cites W1995496629 @default.
- W2079478351 cites W2012183207 @default.
- W2079478351 cites W2032845862 @default.
- W2079478351 cites W2035812197 @default.
- W2079478351 cites W2044433396 @default.
- W2079478351 cites W2044933887 @default.
- W2079478351 cites W2049261064 @default.
- W2079478351 cites W2051503097 @default.
- W2079478351 cites W2053605815 @default.
- W2079478351 cites W2061064038 @default.
- W2079478351 cites W2063183173 @default.
- W2079478351 cites W2064541275 @default.
- W2079478351 cites W2079001764 @default.
- W2079478351 cites W2091080010 @default.
- W2079478351 cites W2097939257 @default.
- W2079478351 cites W2100837269 @default.
- W2079478351 cites W2117071421 @default.
- W2079478351 cites W2123407654 @default.
- W2079478351 cites W2129594505 @default.
- W2079478351 cites W2149025838 @default.
- W2079478351 cites W2149224534 @default.
- W2079478351 cites W2150507107 @default.
- W2079478351 cites W2152851099 @default.
- W2079478351 cites W2155495069 @default.
- W2079478351 cites W2159661209 @default.
- W2079478351 cites W2163863895 @default.
- W2079478351 cites W2264588279 @default.
- W2079478351 cites W2344386688 @default.
- W2079478351 cites W2345060814 @default.
- W2079478351 cites W2416508542 @default.
- W2079478351 cites W247355068 @default.
- W2079478351 doi "https://doi.org/10.1274/jmor.21.134" @default.
- W2079478351 hasPublicationYear "2004" @default.
- W2079478351 type Work @default.
- W2079478351 sameAs 2079478351 @default.
- W2079478351 citedByCount "4" @default.
- W2079478351 countsByYear W20794783512012 @default.
- W2079478351 countsByYear W20794783512018 @default.
- W2079478351 crossrefType "journal-article" @default.
- W2079478351 hasAuthorship W2079478351A5009730530 @default.
- W2079478351 hasAuthorship W2079478351A5054132178 @default.
- W2079478351 hasAuthorship W2079478351A5085907353 @default.
- W2079478351 hasBestOaLocation W20794783511 @default.
- W2079478351 hasConcept C105702510 @default.
- W2079478351 hasConcept C110758877 @default.
- W2079478351 hasConcept C16685009 @default.
- W2079478351 hasConcept C179265757 @default.
- W2079478351 hasConcept C185592680 @default.
- W2079478351 hasConcept C196843134 @default.
- W2079478351 hasConcept C2776690073 @default.
- W2079478351 hasConcept C2777359717 @default.
- W2079478351 hasConcept C2778177303 @default.
- W2079478351 hasConcept C2781087480 @default.
- W2079478351 hasConcept C28748434 @default.
- W2079478351 hasConcept C62525196 @default.
- W2079478351 hasConcept C71924100 @default.
- W2079478351 hasConcept C86803240 @default.
- W2079478351 hasConcept C87073359 @default.
- W2079478351 hasConcept C88972607 @default.
- W2079478351 hasConcept C95444343 @default.
- W2079478351 hasConceptScore W2079478351C105702510 @default.
- W2079478351 hasConceptScore W2079478351C110758877 @default.
- W2079478351 hasConceptScore W2079478351C16685009 @default.
- W2079478351 hasConceptScore W2079478351C179265757 @default.
- W2079478351 hasConceptScore W2079478351C185592680 @default.
- W2079478351 hasConceptScore W2079478351C196843134 @default.
- W2079478351 hasConceptScore W2079478351C2776690073 @default.
- W2079478351 hasConceptScore W2079478351C2777359717 @default.
- W2079478351 hasConceptScore W2079478351C2778177303 @default.
- W2079478351 hasConceptScore W2079478351C2781087480 @default.
- W2079478351 hasConceptScore W2079478351C28748434 @default.
- W2079478351 hasConceptScore W2079478351C62525196 @default.
- W2079478351 hasConceptScore W2079478351C71924100 @default.
- W2079478351 hasConceptScore W2079478351C86803240 @default.
- W2079478351 hasConceptScore W2079478351C87073359 @default.
- W2079478351 hasConceptScore W2079478351C88972607 @default.
- W2079478351 hasConceptScore W2079478351C95444343 @default.
- W2079478351 hasLocation W20794783511 @default.
- W2079478351 hasOpenAccess W2079478351 @default.
- W2079478351 hasPrimaryLocation W20794783511 @default.
- W2079478351 hasRelatedWork W1975714474 @default.
- W2079478351 hasRelatedWork W1984156043 @default.
- W2079478351 hasRelatedWork W1986653738 @default.
- W2079478351 hasRelatedWork W1987902664 @default.
- W2079478351 hasRelatedWork W2004263337 @default.
- W2079478351 hasRelatedWork W2045299582 @default.
- W2079478351 hasRelatedWork W2109904403 @default.
- W2079478351 hasRelatedWork W2366312364 @default.
- W2079478351 hasRelatedWork W2367258775 @default.