Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079488049> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2079488049 endingPage "760" @default.
- W2079488049 startingPage "749" @default.
- W2079488049 abstract "pH gradient HPLC is reported, which is a new original mode of reversed-phase high-performance liquid chromatography applicable to ionogenic analytes. The method consists of programmed increase during the chromatographic run of the eluting strength of the mobile phase with respect to the acid/base analytes separated. Unlike the well-established conventional gradient HPLC, where the eluting power of the mobile phase is increased with time due to the increasing content of organic modifier, in the pH gradient HPLC that is realized by linearly increasing (in the case of acids) or decreasing (in the case of bases) the pH of the eluent of a fixed organic modifier content, thus providing functional increase in the degree of analyte dissociation and, hence, a decrease in its retention. The pH gradient mode has typical features of gradient HPLC, such as reduced peak width and minimized peak-tailing due to peak compression, which is especially advantageous in the case of organic base analytes. It may be of special value for separation of those analytes which are susceptible to the higher concentrations of organic solvents, as many bioanalytes are. A theory of the pH gradient HPLC has been elaborated, and its full mathematical formalistic is presented step by step in a comprehensive manner. Although fundamental relationships at the basis of pH gradient HPLC are more complex than in the case of the organic gradient variant, the resulting mathematical model is easily manageable. Its applicability to predict changes in retention and separation of test mixtures of analytes accompanying the changes in chromatographic conditions has been demonstrated experimentally in both gradient and isocratic HPLC. The proposed model supplies a rational basis for modifications of eluent pH aimed at optimization of separations and for convenient assessment of chromatographically relevant physicochemical parameters of analytes, such as pK(a)." @default.
- W2079488049 created "2016-06-24" @default.
- W2079488049 creator A5011296788 @default.
- W2079488049 creator A5021754483 @default.
- W2079488049 creator A5063747704 @default.
- W2079488049 date "2003-12-31" @default.
- W2079488049 modified "2023-10-15" @default.
- W2079488049 title "pH Gradient Reversed-Phase HPLC" @default.
- W2079488049 cites W1560659985 @default.
- W2079488049 cites W1985721232 @default.
- W2079488049 cites W1990382188 @default.
- W2079488049 cites W2017811176 @default.
- W2079488049 cites W2034171598 @default.
- W2079488049 cites W2034934455 @default.
- W2079488049 cites W2043650752 @default.
- W2079488049 cites W2044898709 @default.
- W2079488049 cites W2045525458 @default.
- W2079488049 cites W2079981234 @default.
- W2079488049 cites W2082092559 @default.
- W2079488049 cites W2096519654 @default.
- W2079488049 cites W2143853833 @default.
- W2079488049 cites W2168149674 @default.
- W2079488049 cites W2187101245 @default.
- W2079488049 cites W2315911157 @default.
- W2079488049 cites W2497735451 @default.
- W2079488049 doi "https://doi.org/10.1021/ac034999v" @default.
- W2079488049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/14750872" @default.
- W2079488049 hasPublicationYear "2003" @default.
- W2079488049 type Work @default.
- W2079488049 sameAs 2079488049 @default.
- W2079488049 citedByCount "56" @default.
- W2079488049 countsByYear W20794880492012 @default.
- W2079488049 countsByYear W20794880492013 @default.
- W2079488049 countsByYear W20794880492014 @default.
- W2079488049 countsByYear W20794880492015 @default.
- W2079488049 countsByYear W20794880492016 @default.
- W2079488049 countsByYear W20794880492017 @default.
- W2079488049 countsByYear W20794880492019 @default.
- W2079488049 countsByYear W20794880492020 @default.
- W2079488049 countsByYear W20794880492021 @default.
- W2079488049 countsByYear W20794880492023 @default.
- W2079488049 crossrefType "journal-article" @default.
- W2079488049 hasAuthorship W2079488049A5011296788 @default.
- W2079488049 hasAuthorship W2079488049A5021754483 @default.
- W2079488049 hasAuthorship W2079488049A5063747704 @default.
- W2079488049 hasConcept C102931765 @default.
- W2079488049 hasConcept C10390740 @default.
- W2079488049 hasConcept C113196181 @default.
- W2079488049 hasConcept C178790620 @default.
- W2079488049 hasConcept C179998833 @default.
- W2079488049 hasConcept C185592680 @default.
- W2079488049 hasConcept C43617362 @default.
- W2079488049 hasConcept C44280652 @default.
- W2079488049 hasConcept C45188465 @default.
- W2079488049 hasConceptScore W2079488049C102931765 @default.
- W2079488049 hasConceptScore W2079488049C10390740 @default.
- W2079488049 hasConceptScore W2079488049C113196181 @default.
- W2079488049 hasConceptScore W2079488049C178790620 @default.
- W2079488049 hasConceptScore W2079488049C179998833 @default.
- W2079488049 hasConceptScore W2079488049C185592680 @default.
- W2079488049 hasConceptScore W2079488049C43617362 @default.
- W2079488049 hasConceptScore W2079488049C44280652 @default.
- W2079488049 hasConceptScore W2079488049C45188465 @default.
- W2079488049 hasIssue "3" @default.
- W2079488049 hasLocation W20794880491 @default.
- W2079488049 hasLocation W20794880492 @default.
- W2079488049 hasOpenAccess W2079488049 @default.
- W2079488049 hasPrimaryLocation W20794880491 @default.
- W2079488049 hasRelatedWork W1981072753 @default.
- W2079488049 hasRelatedWork W2035879625 @default.
- W2079488049 hasRelatedWork W2050532505 @default.
- W2079488049 hasRelatedWork W2052422252 @default.
- W2079488049 hasRelatedWork W2074441509 @default.
- W2079488049 hasRelatedWork W2081849192 @default.
- W2079488049 hasRelatedWork W2094777274 @default.
- W2079488049 hasRelatedWork W57862789 @default.
- W2079488049 hasRelatedWork W67754694 @default.
- W2079488049 hasRelatedWork W2527984489 @default.
- W2079488049 hasVolume "76" @default.
- W2079488049 isParatext "false" @default.
- W2079488049 isRetracted "false" @default.
- W2079488049 magId "2079488049" @default.
- W2079488049 workType "article" @default.