Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079492023> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W2079492023 endingPage "670" @default.
- W2079492023 startingPage "665" @default.
- W2079492023 abstract "The rotifer Brachionus plicatilis is one of the most important food organisms in aquaculture. The resting eggs produced by mictic female rotifers are easily stored and hatched, making them useful as the starter for the mass culture of rotifers in marine larval culture. This study examined the optimum preservation method for resting eggs to ensure a high hatching rate. To produce resting eggs, the marine rotifer B. plicatilis was cultured with Nannochloris oculata (KMMCC 16). The resting eggs were harvested and cryopreserved using 5% and 10% methanol (MeOH), dimethylsulfoxide (DMSO), and glycerol as cryoprotectant agents (CPAs). The cryopreservation comprised slow or rapid freezing and the resting eggs were stored for one month in liquid nitrogen (<TEX>$-196^{circ}C$</TEX>). The resting eggs were also dried at different temperatures (30, 40, and <TEX>$50^{circ}C$</TEX>) and for different times (1, 2, and 3 h). In general, the hatching rates of the resting eggs preserved with CPA were higher than those without CPA and the slow freezing method was better than the rapid freezing method. However, the optimum CPA concentration for the hatching rate of the resting eggs varied with the freezing method and kind of CPA, and the CPA also affected the viability of the resting eggs. Dried resting eggs had a high, rapid hatching rate over 80%. The moisture content of the resting eggs cryopreserved in liquid nitrogen affected the hatching rate. Drying at <TEX>$30^{circ}C$</TEX> for 1 hour resulted in a high hatching rate of the resting eggs. In conclusion, drying at <TEX>$30^{circ}C$</TEX> for 1 hour and preservation in liquid nitrogen with the slow freezing method, without CPA, is recommended for a high hatching rate (ca. 95%) of rotifer resting eggs." @default.
- W2079492023 created "2016-06-24" @default.
- W2079492023 creator A5043260102 @default.
- W2079492023 creator A5067797110 @default.
- W2079492023 date "2011-12-30" @default.
- W2079492023 modified "2023-10-14" @default.
- W2079492023 title "The Hatching Rate of Resting Eggs of the Rotifer Brachionus plicatilis according to Preservation Method" @default.
- W2079492023 cites W1487589808 @default.
- W2079492023 cites W1542940918 @default.
- W2079492023 cites W1966375383 @default.
- W2079492023 cites W1969486071 @default.
- W2079492023 cites W1983821831 @default.
- W2079492023 cites W1987532534 @default.
- W2079492023 cites W1996251399 @default.
- W2079492023 cites W2004654032 @default.
- W2079492023 cites W2021169637 @default.
- W2079492023 cites W2032775884 @default.
- W2079492023 cites W2034691602 @default.
- W2079492023 cites W2035555780 @default.
- W2079492023 cites W2040725140 @default.
- W2079492023 cites W2050386030 @default.
- W2079492023 cites W2064916163 @default.
- W2079492023 cites W2092713296 @default.
- W2079492023 cites W2093781231 @default.
- W2079492023 cites W2330358945 @default.
- W2079492023 cites W2400462198 @default.
- W2079492023 cites W2406067161 @default.
- W2079492023 cites W2465022085 @default.
- W2079492023 cites W2514325717 @default.
- W2079492023 doi "https://doi.org/10.5657/kfas.2011.0665" @default.
- W2079492023 hasPublicationYear "2011" @default.
- W2079492023 type Work @default.
- W2079492023 sameAs 2079492023 @default.
- W2079492023 citedByCount "0" @default.
- W2079492023 crossrefType "journal-article" @default.
- W2079492023 hasAuthorship W2079492023A5043260102 @default.
- W2079492023 hasAuthorship W2079492023A5067797110 @default.
- W2079492023 hasBestOaLocation W20794920231 @default.
- W2079492023 hasConcept C140793950 @default.
- W2079492023 hasConcept C173758957 @default.
- W2079492023 hasConcept C179933525 @default.
- W2079492023 hasConcept C18903297 @default.
- W2079492023 hasConcept C196843134 @default.
- W2079492023 hasConcept C2777181938 @default.
- W2079492023 hasConcept C2777744765 @default.
- W2079492023 hasConcept C2779377512 @default.
- W2079492023 hasConcept C2780881558 @default.
- W2079492023 hasConcept C505870484 @default.
- W2079492023 hasConcept C55493867 @default.
- W2079492023 hasConcept C68528507 @default.
- W2079492023 hasConcept C86803240 @default.
- W2079492023 hasConceptScore W2079492023C140793950 @default.
- W2079492023 hasConceptScore W2079492023C173758957 @default.
- W2079492023 hasConceptScore W2079492023C179933525 @default.
- W2079492023 hasConceptScore W2079492023C18903297 @default.
- W2079492023 hasConceptScore W2079492023C196843134 @default.
- W2079492023 hasConceptScore W2079492023C2777181938 @default.
- W2079492023 hasConceptScore W2079492023C2777744765 @default.
- W2079492023 hasConceptScore W2079492023C2779377512 @default.
- W2079492023 hasConceptScore W2079492023C2780881558 @default.
- W2079492023 hasConceptScore W2079492023C505870484 @default.
- W2079492023 hasConceptScore W2079492023C55493867 @default.
- W2079492023 hasConceptScore W2079492023C68528507 @default.
- W2079492023 hasConceptScore W2079492023C86803240 @default.
- W2079492023 hasIssue "6" @default.
- W2079492023 hasLocation W20794920231 @default.
- W2079492023 hasOpenAccess W2079492023 @default.
- W2079492023 hasPrimaryLocation W20794920231 @default.
- W2079492023 hasRelatedWork W1985764753 @default.
- W2079492023 hasRelatedWork W2034194568 @default.
- W2079492023 hasRelatedWork W2035555780 @default.
- W2079492023 hasRelatedWork W2074419868 @default.
- W2079492023 hasRelatedWork W2078421770 @default.
- W2079492023 hasRelatedWork W2079492023 @default.
- W2079492023 hasRelatedWork W219925537 @default.
- W2079492023 hasRelatedWork W2207114167 @default.
- W2079492023 hasRelatedWork W2523097777 @default.
- W2079492023 hasRelatedWork W2734471538 @default.
- W2079492023 hasVolume "44" @default.
- W2079492023 isParatext "false" @default.
- W2079492023 isRetracted "false" @default.
- W2079492023 magId "2079492023" @default.
- W2079492023 workType "article" @default.