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- W2079492471 abstract "This study investigated whether phenolsulfonphtalein (PR), a common pH indicator in tissue culture media, affects luteinizing hormone (LH) secretion from rat pituitary cells or 17β-estradiol (E2) augmentation of pituitary responsiveness to gonadotropin-releasing hormone (GnRH). PR enhanced GnRH-stimulated LH secretion and shifted the GnRH dose-response curve leftward with a relative potency ratio of 0.24 ± 0.09 (± SE; p < 0.01). The effect of E2 on LH release was significantly diminished by PR, which elevated GnRH-stimulated LH secretion in the absence of E2. This phenomenon was elicited by PR from different sources and was inhibited by the antiestrogen C1628. Thus, PR exerted estrogen-like effects on rat pituitary cells and caused an underestimation of the degree to which E2 enhanced GnRH-stimulated LH secretion." @default.
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- W2079492471 date "1989-01-01" @default.
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- W2079492471 title "Estrogenic effects of phenol red on rat pituitary cell responsiveness to gonadotropin-releasing hormone" @default.
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- W2079492471 doi "https://doi.org/10.1016/0024-3205(89)90264-6" @default.
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