FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079495969> ?p ?o ?g. }

• W2079495969 endingPage "6328" @default.
• W2079495969 startingPage "6324" @default.
• W2079495969 abstract "Intracellular enzymes in erythrocytes can be made accessible for in situ kinetic studies by treating the cells with bifunctional reagents to crosslink proteins, thus creating a network that allows subsequent permeabilization by delipidation without escape of intracellular proteins. Dimethyl suberimidate, dimethyl 3,3'-dithiobispropionimidate, and toluene-2,4-diisocyanate have been used successfully as crosslinking reagents, and digitonin has been used for delipidation. In a systematic study of the in situ behavior of the 11 glycolytic enzymes of rat erythrocytes, it was observed that Km and Vmax values for the majority of the enzymes are essentially the same in situ as in vitro. Lactate dehydrogenase (L-lactate:NAD+ oxidoreductase, EC 1.1.1.27) is inhibited by excess of pyruvate as much in situ as in vitro. Hexokinase (ATP:D-hexose 6-phosphotransferase, EC 2.7.1.1) was allosterically inhibited by glucose 6-phosphate nearly as much in situ as in vitro but was not affected by 2,3-biphosphoglycerate. The allosteric properties of 6-phosphofructokinase (ATP:D-fructose 6-phosphate 1-phosphotransferase, EC 2.7.1.11), glyceraldehyde-phosphate dehydrogenase [D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating), EC 1.2.1.12], and pyruvate kinase (ATP: pyruvate 2-O-phosphotransferase, EC 2.7.1.40) in situ were qualitatively similar to those observed in vitro, but some important quantitative differences were noticed. Particularly striking was the much greater activity of phosphofructokinase in situ compared to that in vitro at physiological concentrations of effector metabolites." @default.
• W2079495969 created "2016-06-24" @default.
• W2079495969 creator A5014909491 @default.
• W2079495969 creator A5035596876 @default.
• W2079495969 creator A5039497467 @default.
• W2079495969 creator A5051074286 @default.
• W2079495969 date "1980-11-01" @default.
• W2079495969 modified "2023-09-26" @default.
• W2079495969 title "Permeabilization of animal cells for kinetic studies of intracellular enzymes: in situ behavior of the glycolytic enzymes of erythrocytes." @default.
• W2079495969 cites W121098377 @default.
• W2079495969 cites W13653301 @default.
• W2079495969 cites W1512022002 @default.
• W2079495969 cites W1514361218 @default.
• W2079495969 cites W1528967283 @default.
• W2079495969 cites W1538274312 @default.
• W2079495969 cites W1583123061 @default.
• W2079495969 cites W1595991577 @default.
• W2079495969 cites W1964283162 @default.
• W2079495969 cites W1965335736 @default.
• W2079495969 cites W1965368241 @default.
• W2079495969 cites W1965793127 @default.
• W2079495969 cites W1967935310 @default.
• W2079495969 cites W2003825794 @default.
• W2079495969 cites W2007613235 @default.
• W2079495969 cites W2009652285 @default.
• W2079495969 cites W2013773193 @default.
• W2079495969 cites W2026239270 @default.
• W2079495969 cites W2070363424 @default.
• W2079495969 cites W2072212474 @default.
• W2079495969 cites W2073214913 @default.
• W2079495969 cites W2074253142 @default.
• W2079495969 cites W2138483175 @default.
• W2079495969 cites W2203178768 @default.
• W2079495969 cites W2402871527 @default.
• W2079495969 cites W2414526517 @default.
• W2079495969 cites W2415076399 @default.
• W2079495969 cites W51072215 @default.
• W2079495969 cites W62266917 @default.
• W2079495969 doi "https://doi.org/10.1073/pnas.77.11.6324" @default.
• W2079495969 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/350276" @default.
• W2079495969 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/6450416" @default.
• W2079495969 hasPublicationYear "1980" @default.
• W2079495969 type Work @default.
• W2079495969 sameAs 2079495969 @default.
• W2079495969 citedByCount "45" @default.
• W2079495969 countsByYear W20794959692013 @default.
• W2079495969 countsByYear W20794959692014 @default.
• W2079495969 countsByYear W20794959692016 @default.
• W2079495969 countsByYear W20794959692022 @default.
• W2079495969 countsByYear W20794959692023 @default.
• W2079495969 crossrefType "journal-article" @default.
• W2079495969 hasAuthorship W2079495969A5014909491 @default.
• W2079495969 hasAuthorship W2079495969A5035596876 @default.
• W2079495969 hasAuthorship W2079495969A5039497467 @default.
• W2079495969 hasAuthorship W2079495969A5051074286 @default.
• W2079495969 hasBestOaLocation W20794959691 @default.
• W2079495969 hasConcept C122245378 @default.
• W2079495969 hasConcept C153911025 @default.
• W2079495969 hasConcept C181199279 @default.
• W2079495969 hasConcept C185592680 @default.
• W2079495969 hasConcept C20251656 @default.
• W2079495969 hasConcept C2776317432 @default.
• W2079495969 hasConcept C2777318727 @default.
• W2079495969 hasConcept C2777504003 @default.
• W2079495969 hasConcept C2780854706 @default.
• W2079495969 hasConcept C2781272698 @default.
• W2079495969 hasConcept C55493867 @default.
• W2079495969 hasConcept C75520062 @default.
• W2079495969 hasConcept C86803240 @default.
• W2079495969 hasConcept C88099638 @default.
• W2079495969 hasConcept C9506865 @default.
• W2079495969 hasConceptScore W2079495969C122245378 @default.
• W2079495969 hasConceptScore W2079495969C153911025 @default.
• W2079495969 hasConceptScore W2079495969C181199279 @default.
• W2079495969 hasConceptScore W2079495969C185592680 @default.
• W2079495969 hasConceptScore W2079495969C20251656 @default.
• W2079495969 hasConceptScore W2079495969C2776317432 @default.
• W2079495969 hasConceptScore W2079495969C2777318727 @default.
• W2079495969 hasConceptScore W2079495969C2777504003 @default.
• W2079495969 hasConceptScore W2079495969C2780854706 @default.
• W2079495969 hasConceptScore W2079495969C2781272698 @default.
• W2079495969 hasConceptScore W2079495969C55493867 @default.
• W2079495969 hasConceptScore W2079495969C75520062 @default.
• W2079495969 hasConceptScore W2079495969C86803240 @default.
• W2079495969 hasConceptScore W2079495969C88099638 @default.
• W2079495969 hasConceptScore W2079495969C9506865 @default.
• W2079495969 hasIssue "11" @default.
• W2079495969 hasLocation W20794959691 @default.
• W2079495969 hasLocation W20794959692 @default.
• W2079495969 hasLocation W20794959693 @default.
• W2079495969 hasLocation W20794959694 @default.
• W2079495969 hasOpenAccess W2079495969 @default.
• W2079495969 hasPrimaryLocation W20794959691 @default.
• W2079495969 hasRelatedWork W1874832133 @default.
• W2079495969 hasRelatedWork W2014184142 @default.
• W2079495969 hasRelatedWork W2028849620 @default.
• W2079495969 hasRelatedWork W2074652316 @default.
• W2079495969 hasRelatedWork W2079495969 @default.

• next