FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2079550023> ?p ?o ?g. }

• W2079550023 endingPage "1033" @default.
• W2079550023 startingPage "1027" @default.
• W2079550023 abstract "Reproducible, rapid measurement of estrogen receptor (ER) binding to DNA was accomplished in microtiter wells treated so that ER-DNA complexes or DNA bound in preference to free ER. Mixtures of 35S-labeled DNA and [3H]estrogen-charged ER ([3H]ER), incubated to equilibrium in microfuge tubes, were transferred to microtiter wells previously treated with histone followed by gelatin. After binding of the DNA or ER-DNA complex to the treated wells, free ER was removed by washing. Radioactivity retained in each well was measured by placing individual wells from snap-apart microtiter plates directly in scintillation fluid. Binding of DNA was saturable, and ER-DNA complex binding was complete within 2 h at 4 C. The use of 35S-labeled DNA and [3H]ER allowed stoichiometric determination of ER bound to DNA. The amount of ER specifically bound to a consensus estrogen-responsive element (ERE) containing the inverted repeat GGTCAgagTGACC was determined by comparing ER bound to plasmid containing or lacking the ERE. At saturating concentrations of ER, plasmids bearing one, two, and four EREs in tandem bound approximately one, two, and four dimeric ER molecules, respectively. Scatchard analysis of saturation binding data revealed a Kd of 0.15 nM for specific ER binding to a single ERE site. Thus, the assay detects ER retaining both DNA-binding and estrogen-binding functions. ER complexed with DNA in the well was also detected using a monoclonal antibody specific for the receptor. Simple modifications of this method would allow study of other DNA-protein interactions." @default.
• W2079550023 created "2016-06-24" @default.
• W2079550023 creator A5005315226 @default.
• W2079550023 creator A5015390672 @default.
• W2079550023 creator A5026307344 @default.
• W2079550023 creator A5042083286 @default.
• W2079550023 creator A5068641693 @default.
• W2079550023 creator A5070205318 @default.
• W2079550023 date "1990-07-01" @default.
• W2079550023 modified "2023-09-24" @default.
• W2079550023 title "A Microtiter Well Assay for Quantitative Measurement of Estrogen Receptor Binding to Estrogen-Responsive Elements" @default.
• W2079550023 cites W135811640 @default.
• W2079550023 cites W1594016728 @default.
• W2079550023 cites W1981686096 @default.
• W2079550023 cites W1982423673 @default.
• W2079550023 cites W1983649348 @default.
• W2079550023 cites W2000429591 @default.
• W2079550023 cites W2001056549 @default.
• W2079550023 cites W2002712696 @default.
• W2079550023 cites W2003716255 @default.
• W2079550023 cites W2006729630 @default.
• W2079550023 cites W2030571151 @default.
• W2079550023 cites W2034889930 @default.
• W2079550023 cites W2035560626 @default.
• W2079550023 cites W2043772857 @default.
• W2079550023 cites W2052909838 @default.
• W2079550023 cites W2066441904 @default.
• W2079550023 cites W2070445591 @default.
• W2079550023 cites W2075788654 @default.
• W2079550023 cites W2144634347 @default.
• W2079550023 cites W2151071712 @default.
• W2079550023 cites W2153550326 @default.
• W2079550023 cites W64285190 @default.
• W2079550023 cites W2117528957 @default.
• W2079550023 doi "https://doi.org/10.1210/mend-4-7-1027" @default.
• W2079550023 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2284005" @default.
• W2079550023 hasPublicationYear "1990" @default.
• W2079550023 type Work @default.
• W2079550023 sameAs 2079550023 @default.
• W2079550023 citedByCount "21" @default.
• W2079550023 countsByYear W20795500232012 @default.
• W2079550023 countsByYear W20795500232015 @default.
• W2079550023 countsByYear W20795500232016 @default.
• W2079550023 countsByYear W20795500232018 @default.
• W2079550023 crossrefType "journal-article" @default.
• W2079550023 hasAuthorship W2079550023A5005315226 @default.
• W2079550023 hasAuthorship W2079550023A5015390672 @default.
• W2079550023 hasAuthorship W2079550023A5026307344 @default.
• W2079550023 hasAuthorship W2079550023A5042083286 @default.
• W2079550023 hasAuthorship W2079550023A5068641693 @default.
• W2079550023 hasAuthorship W2079550023A5070205318 @default.
• W2079550023 hasBestOaLocation W20795500231 @default.
• W2079550023 hasConcept C104317684 @default.
• W2079550023 hasConcept C107824862 @default.
• W2079550023 hasConcept C121608353 @default.
• W2079550023 hasConcept C153911025 @default.
• W2079550023 hasConcept C170493617 @default.
• W2079550023 hasConcept C2777164284 @default.
• W2079550023 hasConcept C2778810118 @default.
• W2079550023 hasConcept C40767141 @default.
• W2079550023 hasConcept C51445715 @default.
• W2079550023 hasConcept C530470458 @default.
• W2079550023 hasConcept C54355233 @default.
• W2079550023 hasConcept C552990157 @default.
• W2079550023 hasConcept C55493867 @default.
• W2079550023 hasConcept C84606932 @default.
• W2079550023 hasConcept C85196483 @default.
• W2079550023 hasConcept C86803240 @default.
• W2079550023 hasConceptScore W2079550023C104317684 @default.
• W2079550023 hasConceptScore W2079550023C107824862 @default.
• W2079550023 hasConceptScore W2079550023C121608353 @default.
• W2079550023 hasConceptScore W2079550023C153911025 @default.
• W2079550023 hasConceptScore W2079550023C170493617 @default.
• W2079550023 hasConceptScore W2079550023C2777164284 @default.
• W2079550023 hasConceptScore W2079550023C2778810118 @default.
• W2079550023 hasConceptScore W2079550023C40767141 @default.
• W2079550023 hasConceptScore W2079550023C51445715 @default.
• W2079550023 hasConceptScore W2079550023C530470458 @default.
• W2079550023 hasConceptScore W2079550023C54355233 @default.
• W2079550023 hasConceptScore W2079550023C552990157 @default.
• W2079550023 hasConceptScore W2079550023C55493867 @default.
• W2079550023 hasConceptScore W2079550023C84606932 @default.
• W2079550023 hasConceptScore W2079550023C85196483 @default.
• W2079550023 hasConceptScore W2079550023C86803240 @default.
• W2079550023 hasIssue "7" @default.
• W2079550023 hasLocation W20795500231 @default.
• W2079550023 hasLocation W20795500232 @default.
• W2079550023 hasOpenAccess W2079550023 @default.
• W2079550023 hasPrimaryLocation W20795500231 @default.
• W2079550023 hasRelatedWork W1517472662 @default.
• W2079550023 hasRelatedWork W1521366099 @default.
• W2079550023 hasRelatedWork W2024832428 @default.
• W2079550023 hasRelatedWork W2058165453 @default.
• W2079550023 hasRelatedWork W2062555839 @default.
• W2079550023 hasRelatedWork W2075786281 @default.
• W2079550023 hasRelatedWork W2079550023 @default.
• W2079550023 hasRelatedWork W2183785214 @default.
• W2079550023 hasRelatedWork W2292672897 @default.

• next