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• W2079556859 abstract "Analysis of NMR relaxation data has provided significant insight on molecular dynamic, leading to a more comprehensive understanding of macromolecular functions. However, traditional methodology allows relaxation measurements performed only at a few fixed high fields, thus severely restricting their potential for extracting more complete dynamic information. Here we report the design and performance of a compact high-speed servo-mechanical shuttle assembly adapted to a commercial 600 MHz high-field superconducting magnet. The assembly is capable of shuttling the sample in a regular NMR tube from the center of the magnet to the top (fringe field ∼0.01 T) in 100 ms with no loss of sensitivity other than that due to intrinsic relaxation. The shuttle device can be installed by a single experienced user in 30 min. Excellent 2D-(15)N-HSQC spectra of (u-(13)C, (15)N)-ubiquitin with relaxation at low fields (3.77 T) and detection at 14.1T were obtained to illustrate its utility in R(1) measurements of macromolecules at low fields. Field-dependent (13)C-R(1) data of (3,3,3-d)-alanine at various field strengths were determined and analyzed to assess CSA and (1)H-(13)C dipolar contributions to the carboxyl (13)C-R(1)." @default.
• W2079556859 created "2016-06-24" @default.
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• W2079556859 date "2012-01-01" @default.
• W2079556859 modified "2023-09-27" @default.
• W2079556859 title "A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR" @default.
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• W2079556859 doi "https://doi.org/10.1016/j.jmr.2011.12.001" @default.
• W2079556859 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22200566" @default.
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